FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438749
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC22170.5053002969807339No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17340.3952145759876376No Hit
TCCGCAAACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAA16200.36923161078429806No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13850.3156702351458351No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13250.3019949903019722No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA12660.28854766620550704No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11360.2589179690438041No Hit
TTCTAGAGGGAGCACCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACC10510.23954470551499832No Hit
CCCCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTAT10160.23156747935607827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10080.2297441133768966No Hit
GTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATAAGCGGT9920.22609738141853314No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9890.22541361917634No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9820.223818173944556No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9680.220627283480988No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT9680.220627283480988No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9220.2101429291006931No Hit
GTCCTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT9040.20604035564753426No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG8830.20125401995218223No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8820.2010260992047845No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8710.19851897098340965No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG8290.18894629959270562No Hit
CTCCTCGACACCGCCTCTGCCTTGTATCGGGAGGCTTTAGAGTCTCCGGA8250.18803461660311477No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT8140.1855274883817399No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7860.17914570745460387No Hit
ACCACGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATCTTCCTGCTGGT7510.17116848129568385No Hit
CTTCAAGATCTCCTCGACACCGCCTCTGCCTTGTATCGGGAGGCTTTAGA7140.16273541364196842No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7020.16000036467319584No Hit
GGTTATTCTTCCAAACCTCGACGAGGCATGGGGACGAATCTTTCTGTTCC6860.1563536327148324No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6830.15566987047263925No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA6570.14974393104029868No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6560.14951601029290093No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC6560.14951601029290093No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT6490.14792056506111695No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6470.1474647235663215No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6460.1472368028189238No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6380.1454134368397421No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG6370.14518551609234437No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6360.14495759534494665No Hit
GTTATTCTTCCAAACCTCGACGAGGCATGGGGACGAATCTTTCTGTTCCC6320.1440459123553558No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6250.1424504671235718No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6200.14131086338658322No Hit
CCCAAGGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG6100.13903165591260608No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6070.13834789367041292No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTATAGACCACCAAATGCC5950.13561284470164037No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGCA5930.1351570032068449No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5920.1349290824594472No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5870.13378947872245864No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5850.1333336372276632No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5830.13287779573286776No Hit
CTCCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5810.13242195423807235No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5770.13151027124848147No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5760.13128235050108378No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5710.1301427467640952No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5660.12900314302710664No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC5610.12786353929011804No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5580.1271797770479249No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5500.12535641106874318No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5500.12535641106874318No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG5380.1226213620999706No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT5300.12079799612078887No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5290.12057007537339118No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5280.12034215462599346No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5260.11988631313119803No Hit
GTATGGATCGGTAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5200.11851878864681173No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5180.1180629471520163No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA5150.11737918490982316No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCT5140.11715126416242544No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5070.11555581893064144No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGAGGGAGCACCCACG5070.11555581893064144No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5050.11509997743584602No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCGAGGGGGAGCACCCACG5010.11418829444625514No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5000.11396037369885745No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5000.11396037369885745No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4960.11304869070926657No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4940.11259284921447114No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4920.11213700771967572No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4910.111909086972278No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA4820.10985780024569856No Hit
GCAGACGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCC4790.1091740380035054No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4750.10826235501391457No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTC4700.10712275127692598No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4680.10666690978213056No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4670.10643898903473284No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4660.10621106828733512No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4650.10598314753993741No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4630.10552730604514199No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT4610.10507146455034655No Hit
GTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGCTCCGCA4570.1041597815607557No Hit
CACATAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG4500.10256433632897168No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4490.10233641558157398No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4470.10188057408677856No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4420.10074097034978999No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4390.10005720810759683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)