FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438749
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC16960.3865535875865244No Hit
TCCGCAAACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAA15170.34575577380233347No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14410.3284337970001071No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13900.31680983888282366No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12970.29561320937483615No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11410.26005757278079267No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA11200.25527123708544064No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10510.23954470551499832No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10050.22906035113470347No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9840.22427401543935144No Hit
TTCTAGAGGGAGCACCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACC9690.2208552042283857No Hit
GTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATAAGCGGT9660.22017144198619257No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9240.21059877059548854No Hit
CCCCCTCGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTAT9220.2101429291006931No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT9080.2069520386371251No Hit
CTCCTCGACACCGCCTCTGCCTTGTATCGGGAGGCTTTAGAGTCTCCGGA9030.20581243490013654No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG8960.2042169896683525No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8840.20148194069957998No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG8470.1930488730458645No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8450.19259303155106908No Hit
GTCCTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT8420.19190926930887592No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7920.1805132319389902No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT7740.17641065848583132No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7350.16752174933732042No Hit
ACCACGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATCTTCCTGCTGGT7300.16638214560033185No Hit
CTTCAAGATCTCCTCGACACCGCCTCTGCCTTGTATCGGGAGGCTTTAGA7230.16478670036854784No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7160.16319125513676386No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7020.16000036467319584No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA6970.15886076093620727No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT6940.1581769986940141No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6830.15566987047263925No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6710.15293482150386667No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6630.15111145552468497No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6350.14472967459754893No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6350.14472967459754893No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6330.14427383310275352No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG6310.14381799160795808No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6240.1422225463761741No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6150.14017125964959465No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6100.13903165591260608No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6080.13857581441781064No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG6050.1378920521756175No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5970.13606868619643578No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5840.13310571648026548No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5790.1319661127432769No Hit
CCCAAGGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG5780.1317381919958792No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTATAGACCACCAAATGCC5780.1317381919958792No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5760.13128235050108378No Hit
CTCCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5670.12923106377450433No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5640.12854730153231117No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGAGGGAGCACCCACG5620.12809146003751576No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5600.12763561854272032No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT5580.1271797770479249No Hit
GGTTATTCTTCCAAACCTCGACGAGGCATGGGGACGAATCTTTCTGTTCC5560.12672393555312947No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5490.12512849032134546No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5390.12284928284736832No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5370.1223934413525729No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGCA5350.12193759985777745No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5330.12148175836298203No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC5300.12079799612078887No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCT5250.1196583923838003No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5240.11943047163640258No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5220.11897463014160715No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG5170.11783502640461858No Hit
GTTATTCTTCCAAACCTCGACGAGGCATGGGGACGAATCTTTCTGTTCCC5130.11692334341502773No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC5120.11669542266763001No Hit
GTATGGATCGGTAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5030.11464413594105057No Hit
GTCTATAAGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC4920.11213700771967572No Hit
CTTTTACACAATGTGGTTATCCTGCCTTGATGCCTTTATATGCATGTATA4900.11168116622488028No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCGAGGGGGAGCACCCACG4890.11145324547748257No Hit
CTCATCTGCCGGTCCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGG4890.11145324547748257No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4880.11122532473008485No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4870.11099740398268713No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4850.11054156248789171No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4850.11054156248789171No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT4840.110313641740494No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGGGCAGCA4840.110313641740494No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4780.10894611725610771No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4700.10712275127692598No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4690.10689483052952826No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4670.10643898903473284No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4670.10643898903473284No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4670.10643898903473284No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4540.10347601931856255No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4510.10279225707636941No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4480.10210849483417625No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4460.10165265333938082No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4430.10096889109718768No Hit
GGAAGATATAGGCCCCTCACTCTGGGATCTAGCAGAGCGTGGTGGAATGT4390.10005720810759683No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA4390.10005720810759683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)