FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129068
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9310.7213251929215607No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9170.7104781975392817No Hit
CACAAAAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG8720.6756128552390989No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7760.601233458332042No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7480.5795394675674838No Hit
CAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG6740.5222053491182942No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5340.4137353952955032No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4520.3502029937707255No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4470.34632906684848297No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3730.2889949483992934No Hit
GCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG3450.26730095763473516No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3370.2611026745591471No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3340.2587783184058016No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT3270.25335482071466203No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3250.25180524994576503No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3060.23708432764124338No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT2980.2308860445656553No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA2970.2301112591812068No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC2880.22313819072117022No Hit
GCTTTTGTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG2820.2184894784144792No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC2670.2068676976477516No Hit
GTAGAGGCCCAAACGGCCCCGAGATGGGTCGTCCGCGGGATTCAGCGCCG2660.20609291226330304No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2630.20376855610995753No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC2600.201444199956612No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG2570.19911984380326647No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC2520.19524591688102397No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG2520.19524591688102397No Hit
CGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCA2470.1913719899587814No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG2380.18439892149874484No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2360.18284935072984781No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT2350.18207456534539934No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA2240.17355192611646575No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2170.1681284284253262No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA2150.16657885765642916No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC2140.16580407227198066No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT2120.16425450150308363No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT2090.16193014534973812No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGAAGAAGGAGG2060.1596057891963926No Hit
GGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG2050.1588310038119441No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA2040.15805621842749557No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC2030.1572814330430471No Hit
GTATACATGCATATAATGGCATCAAGGCAGGATAGCCACATTGTGTAAAA2010.15573186227415006No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC2000.15495707688970156No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA1940.1503083645830105No Hit
GACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC1940.1503083645830105No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG1940.1503083645830105No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA1930.149533579198562No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG1910.14798400842966497No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG1900.14720922304521647No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC1880.14565965227631947No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC1840.14256051073852544No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT1830.1417857253540769No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA1790.1386865838162829No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAAG1770.13713701304738588No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC1760.13636222766293735No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT1740.13481265689404034No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG1720.13326308612514332No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGT1710.13248830074069484No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG1710.13248830074069484No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT1700.1317135153562463No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT1690.1309387299717978No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT1660.12861437381845228No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1640.12706480304955528No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA1620.12551523228065825No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA1620.12551523228065825No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG1610.12474044689620976No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG1600.12396566151176125No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG1600.12396566151176125No Hit
AAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGAAGAAGGAGGGGA1580.12241609074286422No Hit
ACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG1560.12086651997396722No Hit
ACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT1530.11854216382062169No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCATTATATGCATGTATA1530.11854216382062169No Hit
GAGATGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC1530.11854216382062169No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC1520.11776737843617317No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT1490.11544302228282766No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA1480.11466823689837914No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG1460.11311866612948214No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCGAGCAGCC1450.11234388074503363No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA1440.11156909536058511No Hit
CTCTAAGGCCTCCCGGTACAGAGCAGAGGCGGTGTCGAGGAGGTCTCGAA1430.11079430997613662No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA1430.11079430997613662No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT1420.11001952459168811No Hit
ACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGC1420.11001952459168811No Hit
GGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCG1410.1092447392072396No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG1400.1084699538227911No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG1400.1084699538227911No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA1390.10769516843834258No Hit
AGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAA1380.10692038305389406No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA1370.10614559766944556No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA1370.10614559766944556No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1360.10537081228499706No Hit
ACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACC1360.10537081228499706No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA1340.10382124151610003No Hit
CCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAG1340.10382124151610003No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC1330.10304645613165152No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC1330.10304645613165152No Hit
TCCCGGTACAGAGCAGAGGCGGTGTCGAGGAGGTCTCGAATAGAAGGAAA1320.10227167074720303No Hit
AACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT1320.10227167074720303No Hit
CACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG1320.10227167074720303No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC1310.10149688536275452No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG1300.100722099978306No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA1300.100722099978306No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA1300.100722099978306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)