FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402289
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15830.3934982065132281No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG14590.36267459463221713No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12720.3161905992955313No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT11460.2848698323841815No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10920.2714466465650316No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10790.2682151388678289No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10600.2634921660796094No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9970.24783178262393454No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9670.24037445716885128No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9420.2341600192896152No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9090.22595696128902354No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8920.221731143531143No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8650.21501955062156805No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8400.20880511274233202No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8210.2040821399541126No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8150.20259067486309593No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAAGCC8120.20184494231758762No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7650.1901617991046238No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7180.17847865589165998No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6980.17350710558827112No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6940.17251279552759335No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAGGCTCTGCTAG6910.17176706298208502No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6730.16729266770903506No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6500.16157538486013787No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA6430.15983534225395174No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6360.15809529964776564No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6170.15337232685954627No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6140.1526265943140379No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5970.14840077655615738No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5890.14641215643480185No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGGCAGGTC5840.14516926885895465No Hit
GGATAGGGGCATTTGGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACT5800.14417495879827685No Hit
CCTTTACCCCGTTGCCCGGCAACGGGCAGGTCTTTGCCAAGTGTTTGCTG5560.13820909843421023No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA5550.1379605209190408No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5500.1367176333431936No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5460.1357233232825158No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCCTGGTGGAATGTTGTGG5450.13547474576734636No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG5350.13298897061565193No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5250.1305031954639575No Hit
TCTGTGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG5210.12950888540327973No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG5170.12851457534260197No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCTTTGTAAGTTGGCGA5100.12677453273641587No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT5060.12578022267573807No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGCAAACTGC5050.12553164516056864No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5030.12503449013022977No Hit
CCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4890.12155440491785756No Hit
ATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT4820.11981436231167145No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4810.11956578479650201No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4760.11832289722065481No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4710.1170800096448076No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4710.1170800096448076No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4690.1165828546144687No Hit
CTCTGGGATCTAGCAGAGCCTGGTGGAATGTTGTGGAGTTCCACTGCATG4670.11608569958412981No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4630.11509138952345203No Hit
GAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA4500.11185988182624929No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4460.1108655717655715No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4460.1108655717655715No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4450.11061699425040207No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4390.10912552915938542No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4370.10862837412904654No Hit
ATGTAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTTTCCATGC4370.10862837412904654No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4350.10813121909870765No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4340.10788264158353819No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA4340.10788264158353819No Hit
CCCCAAAAGACCGCCGTGTGGAGGGGTGAACCCTAGTCCGAATGCTCCCG4330.10763406406836876No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4320.10738548655319932No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4290.10663975400769098No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4260.10589402146218266No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4240.10539686643184376No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4230.10514828891667434No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4200.10440255637116601No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4180.10390540134082712No Hit
GAATACAAGTGCAGTTTGCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4160.10340824631048823No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4110.10216535873464103No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4080.1014196261891327No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4050.10067389364362435No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4030.10017673861328548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)