FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460729
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC20600.4471175029138604No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18530.4021887052909628No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG15830.3435859257828354No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13870.30104464880656523No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA12730.2763012530142448No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12160.2639295551180846No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11210.24331005862448424No Hit
CCTCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11190.24287596396146108No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10450.22681446142960396No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10320.22399284611995338No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT10280.22312465679390706No Hit
CCTCCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10170.22073713614727963No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9970.21639618951704798No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9860.21400866887042058No Hit
CTCCTCCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9850.213791621538909No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG9430.2046756336154225No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9400.20402449162088776No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9350.20293925496332987No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC9240.20055173431670242No Hit
GTATACATGCATATAAAGGCATCAAGGCTGGATATCCACATTGTGTAAAA9210.1999005923221677No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG8860.1923039357192623No Hit
GTTGTCAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8830.19165279372472754No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG8400.18231975846972948No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8300.18014928515461365No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA8300.18014928515461365No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7790.16907987124752294No Hit
GGTTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA7640.16582416127484922No Hit
GTGGCTATCCAGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG7590.1647389246172913No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7480.1623514039706639No Hit
ACTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7220.15670817335136272No Hit
GGATATCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAAT7090.15388655804171217No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7050.15301836871566582No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT6930.15041380073752683No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6930.15041380073752683No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6900.14976265874299208No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6890.1495456114114805No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA6810.14780923275938784No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6700.14542171211276042No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6680.14498761744973726No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6510.14129781281404036No Hit
CCTTTTACACAATGTGGATATCCAGCCTTGATGCCTTTATATGCATGTAT6490.14086371815101717No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6480.1406466708195056No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6170.13391820354264655No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6160.13370115621113496No Hit
CATCTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6090.13218182489055388No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6090.13218182489055388No Hit
GATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC6050.13131363556450756No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6050.13131363556450756No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5980.12979430424392646No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5960.1293602095809033No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5960.1293602095809033No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5940.12892611491788014No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5900.12805792559183382No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGC5880.12762383092881066No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5860.12718973626578747No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5850.1269726889342759No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5850.1269726889342759No Hit
GTATACATCTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC5850.1269726889342759No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC5840.1267556416027643No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5820.12632154693974113No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5750.12480221561916008No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5650.12263174230404425No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5530.12002717432590526No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5500.11937603233137051No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5380.11677146435323152No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5360.11633736969020834No Hit
GTGGATATCCAGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG5350.11612032235869676No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5350.11612032235869676No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCC5330.1156862276956736No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5310.11525213303265043No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5280.11460099103811569No Hit
TACCTTGGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5280.11460099103811569No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5260.11416689637509252No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC5250.11394984904358095No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGAACGTCC5180.11243051772299986No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5160.11199642305997669No Hit
CATATAAAGGCATCAAGGCTGGATATCCACATTGTGTAAAAGGAGCAGCA5140.11156232839695353No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGACTTCCAAAC5030.10917480775032612No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5020.10895776041881453No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5010.10874071308730295No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4990.10830661842427979No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4970.10787252376125661No Hit
CTCCTGCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4900.10635319244067552No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC4900.10635319244067552No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4900.10635319244067552No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4870.10570205044614078No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAGTGGATTCCACTG4860.10548500311462922No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAGATGTATA4860.10548500311462922No Hit
GATATCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATT4850.10526795578311762No Hit
CTATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4820.10461681378858287No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4820.10461681378858287No Hit
TATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATG4810.1043997664570713No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4810.1043997664570713No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4790.10396567179404813No Hit
GGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC4790.10396567179404813No Hit
GTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC4780.10374862446253655No Hit
GCCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4750.1030974824680018No Hit
CTTCTGGACTGCCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4750.1030974824680018No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4730.10266338780497863No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT4720.10244634047346704No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4720.10244634047346704No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4710.10222929314195547No Hit
GTTCAGATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4700.10201224581044388No Hit
GGTGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC4700.10201224581044388No Hit
AAGGTGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA4690.1017951984789323No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4680.10157815114742073No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4680.10157815114742073No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4670.10136110381590913No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4660.10114405648439756No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4640.10070996182137439No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4620.10027586715835121No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4620.10027586715835121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)