FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460729
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17920.38894881806875625No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17340.3763600728410844No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC17240.37418959952596864No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14620.31732319866993397No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA12520.2717432590525016No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11670.25329423587401706No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10990.23853501733122942No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10910.23679863867913675No Hit
CCTCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10810.23462816536402092No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT10770.23375997603797458No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10240.2222564674678607No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10240.2222564674678607No Hit
GTATACATGCATATAAAGGCATCAAGGCTGGATATCCACATTGTGTAAAA9760.21183819555530475No Hit
CCTCCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9360.20315630229484147No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9340.20272220763181825No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9330.20250516030030666No Hit
CTCCTCCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9030.1959937403549592No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8850.19208688838775073No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC8530.1851413737793801No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG8520.1849243264478685No Hit
GTTGTCAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8380.18188566380670634No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8370.18166861647519475No Hit
GGTTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA8170.1773276698449631No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG8090.17559129119287042No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG8090.17559129119287042No Hit
GTGGCTATCCAGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG7860.17059920256810404No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7780.16886282391601135No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7480.1623514039706639No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7380.16018093065554806No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGAACGTCC7170.1556229366938048No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA7140.15497179469927008No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7050.15301836871566582No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7000.1519331320581079No Hit
GGATATCCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAAT6930.15041380073752683No Hit
CCTTTTACACAATGTGGATATCCAGCCTTGATGCCTTTATATGCATGTAT6750.14650694877031833No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6680.14498761744973726No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT6670.1447705701182257No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6600.1432512387976446No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6520.14151486014555195No Hit
GATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC6330.13739096084683186No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6300.13673981885229713No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG6280.13630572418927395No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6280.13630572418927395No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6280.13630572418927395No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC6230.13522048753171603No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6220.13500344020020447No Hit
ACTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6150.1334841088796234No Hit
CATCTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6110.13261591955357704No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6100.13239887222206548No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6070.13174773022753072No Hit
GTATACATCTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC5940.12892611491788014No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5920.12849202025485698No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5920.12849202025485698No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5880.12762383092881066No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5870.12740678359729904No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5840.1267556416027643No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5800.125887452276718No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5790.1256704049452064No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5780.12545335761369483No Hit
CTATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5740.12458516828764848No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5690.12349993163009057No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5680.12328288429857899No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA5660.12284878963555583No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCC5590.12132945831497474No Hit
GTGGATATCCAGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG5410.11742260634776626No Hit
GTTCAGATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5370.11655441702171992No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5270.1143839437066041No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5260.11416689637509252No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5250.11394984904358095No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5240.11373280171206934No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5230.11351575438055778No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5210.1130816597175346No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5210.1130816597175346No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5150.1117793757284651No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5140.11156232839695353No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5110.11091118640241877No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCT5080.11026004440788403No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG5000.10852366575579135No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGC4990.10830661842427979No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4970.10787252376125661No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGACTTCCAAAC4960.10765547642974505No Hit
GTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGC4950.10743842909823344No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4950.10743842909823344No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4910.10657023977218713No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4910.10657023977218713No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4900.10635319244067552No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4870.10570205044614078No Hit
GGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC4840.10505090845160604No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT4820.10461681378858287No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4820.10461681378858287No Hit
TACCTTGGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4810.1043997664570713No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4810.1043997664570713No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC4810.1043997664570713No Hit
TCCTCAGGCCATACAGTGGAATCCACTTCCTGCTGGTGGCTCCAGTTCCG4790.10396567179404813No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC4730.10266338780497863No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4730.10266338780497863No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4710.10222929314195547No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4700.10201224581044388No Hit
CTCCTGCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4680.10157815114742073No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4670.10136110381590913No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG4650.10092700915288597No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4640.10070996182137439No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTC4640.10070996182137439No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4620.10027586715835121No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4620.10027586715835121No Hit
CATATAAAGGCATCAAGGCTGGATATCCACATTGTGTAAAAGGAGCAGCA4610.10005881982683965No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAGATGTATA4610.10005881982683965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)