FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115130
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6200.5385216711543472No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4290.37262225310518543No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3460.3005298358377486No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA3180.27620950230174585No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC2790.24233475201945626No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG2640.22930600191088335No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT2630.22843741857031183No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2610.22670025188916876No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT2570.22322591852688267No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA2470.21454008512116737No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT2310.20064275167202295No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT2290.19890558499087987No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA2270.1971684183097368No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG2210.19195691826630765No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG2090.1815339181794493No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT2060.17892816815773474No Hit
CTACTATTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG2050.17805958481716322No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT2030.17632241813602015No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA1990.17284808477373403No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG1980.1719795014331625No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC1970.171110918092591No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA1960.17024233475201944No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGAACTTTACCACAG1940.1685051680708764No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA1930.16763658473030488No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA1890.16416225136801876No Hit
CAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG1830.15895075132458958No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG1830.15895075132458958No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA1810.15721358464344654No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC1780.15460783462173194No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC1760.1528706679405889No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA1720.1493963345783028No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC1680.14592200121601667No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA1640.14244766785373056No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA1620.14071050117258752No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC1600.13897333449144447No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG1550.1346304177885868No Hit
ATATGTTCCTGTGGTAAAGTTCCCCAACTTCCAATTACATATCCCATGAA1530.13289325110744377No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG1510.1311560844263007No Hit
ACTGATGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTC1490.12941891774515765No Hit
CCTAATCTCCTCCCCCTGCTCCTCCCAGTCTTTAAACAAACAGTCTTTGA1480.1285503344045861No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG1470.1276817510640146No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA1460.12681316772344306No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT1450.12594458438287154No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCC1440.1250760010423No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC1440.1250760010423No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGACAACGGTCAGGTC1380.11986450099887083No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA1380.11986450099887083No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG1370.11899591765829932No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA1330.11552158429601321No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT1330.11552158429601321No Hit
CCTTTACCCCGTTGCCCGACAACGGTCAGGTCTCTGCCAAGTGTTTGCTG1330.11552158429601321No Hit
ATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA1320.11465300095544168No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT1300.11291583427429862No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1300.11291583427429862No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG1280.11117866759315556No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG1280.11117866759315556No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA1280.11117866759315556No Hit
ATCACCAGTTGGACCCTGCGTTCGGAGCCAACTCAAACAATCCAGATTGG1270.11031008425258404No Hit
GTGCACACGGTCCGGCAGGTGAGAAGGCACAGACGGGGAGACCGCGTAAA1270.11031008425258404No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG1270.11031008425258404No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGGAGTTCCACTGCATG1260.1094415009120125No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT1260.1094415009120125No Hit
GATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA1260.1094415009120125No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC1240.10770433423086946No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC1240.10770433423086946No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC1240.10770433423086946No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG1230.10683575089029793No Hit
GTCCGGCAGGTGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC1220.10596716754972639No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC1200.10423000086858335No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC1200.10423000086858335No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG1200.10423000086858335No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC1190.10336141752801181No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT1190.10336141752801181No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTGGGCTGTCT1190.10336141752801181No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG1180.10249283418744029No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA1170.10162425084686875No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC1170.10162425084686875No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC1170.10162425084686875No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA1160.10075566750629722No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC1160.10075566750629722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)