FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137586
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8720.6337854142136554No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5710.41501315540825373No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4630.33651679676711294No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4270.3103513438867327No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4250.3088977076156004No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4090.29726861744654254No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG3550.25802043812597214No Hit
GTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCTAAGC3470.2522058930414432No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3350.24348407541464975No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT3330.2420304391435175No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA3020.21949907694096782No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT2870.20859680490747604No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG2720.19769453287398425No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG2680.19478726033171978No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA2590.18824589711162473No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA2540.18461180643379413No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA2520.18315817016266192No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG2490.18097771575596355No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT2390.17370953440030235No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA2370.1722558981291701No Hit
ATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATATT2360.171529079993604No Hit
CGGTGGTCGCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2350.17080226185803787No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG2270.16498771677350893No Hit
GTACTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTCAATAGACCTAT2220.16135362609567833No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC2220.16135362609567833No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2170.15771953541784775No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG2170.15771953541784775No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT2160.15699271728228162No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA2110.15335862660445104No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2110.15335862660445104No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2040.1482708996554882No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG2040.1482708996554882No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTTCTGCCTCCACCAATCGGCAGT2010.14609044524878986No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2000.14536362711322373No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA1980.1439099908420915No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT1970.14318317270652536No Hit
GAAACTTTACTGGGCTTTATTCTTCTACTGTACCTATCTTTAATCCTGAG1920.1395490820286948No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT1790.13010044626633524No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACACTGTGTAAAA1790.13010044626633524No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACTGGG1780.1293736281307691No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA1770.128646809995203No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC1760.12791999185963687No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA1760.12791999185963687No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG1750.12719317372407077No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC1710.12428590118180628No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT1710.12428590118180628No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT1700.12355908304624016No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG1700.12355908304624016No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG1700.12355908304624016No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC1660.1206518105039757No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC1650.11992499236840956No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG1640.11919817423284346No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC1590.11556408355501288No Hit
GACCACTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC1590.11556408355501288No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA1580.11483726541944674No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG1540.11192999287718228No Hit
CCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTT1530.11120317474161616No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA1530.11120317474161616No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG1500.1090227203349178No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG1500.1090227203349178No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT1490.10829590219935167No Hit
CTCCCATAGGAATCTTACGAAAGCCCAGGATGATGGGATGGGAATACAAG1490.10829590219935167No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGGAGTTCCACTGCATG1480.10756908406378556No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA1470.10684226592821945No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG1470.10684226592821945No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA1460.1061154477926533No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT1450.1053886296570872No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1440.10466181152152108No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA1430.10393499338595498No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA1430.10393499338595498No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC1430.10393499338595498No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT1430.10393499338595498No Hit
CCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATT1430.10393499338595498No Hit
TTGGTTCGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAG1400.10175453897925663No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG1400.10175453897925663No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA1390.10102772084369048No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA1380.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)