FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482716
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN60081.2446241682479966No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC14740.305355529959645No Hit
TCCATAGAGTGTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACC10830.22435552167319917No Hit
GTGTGGTTATCCTGCTTTAATGCCATTGTATGCCTGTATTCAATCTAAGC10750.22269823250109796No Hit
GTGTAAATAGTGTCTGGTTTGGAAGTAATGATTAACTACCTGTTCTGGAT8510.17629413568226454No Hit
CTTTTACACAGTGTGGTTATCCTGCTTTAATGCCATTGTATGCCTGTATT8160.1690434955543218No Hit
AATGTAAGGAGTAGCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA7890.16345014459848026No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT7330.1518491203937719No Hit
GTGTCGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7190.1489488643425948No Hit
CCTTACATTTTATGGGGTATGTCATTGGAAGTTATGGGTCCTTGCCACAA7030.14563428599839243No Hit
CCTTAAATTTTATGGGGTATGTCATTGGAAGTTATGGGTCCTTGCCACAA6840.14169822421465209No Hit
AATTTAAGGAGTAGCCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA6480.13424042294019672No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6280.13009720000994374No Hit
CCTTACATTTTATGGGGTATGTCATTGGAAGTTATGGGTCTTTGCCACAA6170.1278184273983046No Hit
ATATCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAG5890.12201791529595041No Hit
CCTAATACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTC5720.11849617580523537No Hit
CAATTCGTTGACACACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTT5660.11725320892615948No Hit
GGATAAGGGCATTAAACCTTATTATCCAGAACAGGTAGTTAATCATTACT5220.10813811847960292No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5030.10420205669586258No Hit
CTCTATAACTATATCTCTTCCAACCATAAGACAAGAAATGTGAAACCACA5000.10358057325632462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)