FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357892
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52001.452952287282197No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9270.25901668659819166No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT7760.21682518748672783No Hit
CATCTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT7180.20061918120550334No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT7100.19838386999429997No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6440.17994255250187208No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6270.1751925161780649No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6070.16960423815005646No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC6050.1690454103472556No Hit
GTATACATCTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC5920.1654130296290501No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT5880.1642953740234484No Hit
CCTCCAGCCTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5790.16178064891084462No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATCTAA5630.15731002648843787No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5560.1553541291786349No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC5210.1455746426296201No Hit
GTCTAAAGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4940.1380304672918087No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA4890.1366333977848066No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT4590.12825098074279392No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA4430.12378035832038714No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG4420.12350094441898674No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC4390.12266270271478547No Hit
GGGTATACATCTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA4250.11875090809517956No Hit
GTTTCCCTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATG4190.11707442468677702No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGG4190.11707442468677702No Hit
CACTAAATATTTGCCTTTAGACAAAGGAATTAAACCTTATTATCCAGAAC4160.11623618298257576No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA4130.11539794127837448No Hit
CATGTATACAAGCCAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4010.11204497446156941No Hit
ATTCTATCCTACCCACACTAAATATTTGCCTTTAGACAAAGGAATTAAAC3920.1095302493489656No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA3900.10897142154616478No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA3870.1081331798419635No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCTCACCCGTG3820.10673611033496137No Hit
CAAATTGTTGACACCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGA3810.10645669643356097No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG3790.10589786863076012No Hit
AGATACATGAAACCACAATAGTTGCCTGATCTTTAAACCCATGTTAGTAT3750.10478021302515843No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA3720.10394197132095717No Hit
AGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATACA3640.1017066601097538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)