FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357892
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN51921.4507169760709935No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG9160.255943133682787No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT7320.20453097582510926No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT7120.19894269779710078No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG6720.18776614174108391No Hit
CATCTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6550.18301610541727673No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6520.18217786371307546No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT6400.17882489689627037No Hit
CCTCCAGCCTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6260.17491310227666446No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6120.17100130765705857No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATCTAA5850.16345713231924716No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5790.16178064891084462No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA5730.16010416550244208No Hit
GTCTAAAGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5570.15563354308003532No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5410.15116292065762857No Hit
GTATACATCTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC5380.1503246789534273No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC4740.13244218926380025No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG4730.13216277536239984No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACATGTTCTGG4660.13020687805259687No Hit
GTTTCCCTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATG4350.12154504710918378No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGCTCACCCGTG4320.1207068054049825No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACA4290.11986856370078125No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT4180.11679501078537659No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC4180.11679501078537659No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG4160.11623618298257576No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4160.11623618298257576No Hit
AGATACATGAAACCACAATAGTTGCCTGATCTTTAAACCCATGTTAGTAT4040.11288321616577067No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA4000.11176556056016898No Hit
GGGTATACATCTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA3740.104500799123758No Hit
AGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATACA3730.10422138522235758No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3700.1033831435181563No Hit
CACTAAATATTTGCCTTTAGACAAAGGAATTAAACCTTATTATCCAGAAC3630.10142724620835336No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA3630.10142724620835336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)