FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455070
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28700.6306722042762651No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24230.5324455578262685No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT12560.276001494275606No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11410.2507306568220274No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10960.24084206825323576No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT10730.23578790076252007No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10320.22677829784428769No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10290.22611905860636825No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10100.2219438767662118No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9480.20831959918254334No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9350.20546289581822577No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC8660.19030039334607862No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8410.18480673303008327No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8360.18370800096688422No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8160.17931307271408795No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7940.17447865163601203No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7700.1692047377326565No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGTCAGTC7570.16634803436833895No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7470.1641505702419408No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCAGT7220.15865690992594544No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC7120.15645944579954732No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT6990.15360274243522973No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6820.14986705342035292No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6700.14723009646867513No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6650.14613136440547608No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6230.1369020150746039No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6050.13294657964708728No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5890.12943063704485025No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5830.12811215856901137No Hit
GTTCCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA5700.12525545520469378No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5630.1237172303162151No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5430.11932230206341882No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5410.11888280923813918No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5370.11800382358757994No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5370.11800382358757994No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5270.11580635946118178No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT5170.11360889533478366No Hit
TCCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATT5170.11360889533478366No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5080.11163117762102534No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC5050.1109719383831059No Hit
CTTTTACACAATGTGGGTATCCTGCTTTAATGCCGTTGTATGCATGTATT4970.10921396708198738No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4970.10921396708198738No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACAACC4940.10855472784406794No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA4890.10745599578086888No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4870.10701650295558926No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4830.10613751730503No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4780.10503878524183091No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4770.10481903882919111No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4710.10350056035335223No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4680.10284132111543279No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4650.10218208187751335No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4620.10152284263959391No Hit
ATATTAGTGTTAACATAACTTACTACTAGGTCCCTGGATGTTGGATCGTC4590.10086360340167448No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4570.10042411057639485No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4570.10042411057639485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)