FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455070
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28660.6297932186257059No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21670.4761904761904762No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT12190.2678708770079329No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11660.25622431713802274No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11310.24853319269562923No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10550.2318324653350034No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10260.2254598193684488No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG10210.22436108730524976No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT10190.22392159447997012No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9940.21842793416397477No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9890.2173292021007757No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9580.21051706330894146No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9530.20941833124574238No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC9060.19909024985167115No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC8160.17931307271408795No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC7930.17425890522337223No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7810.17162194827169447No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7620.167446766431538No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7580.16656778078097875No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT7240.15909640275122508No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGTCAGTC7000.15382248884786956No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCAGT6690.14701035005603533No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6450.1417364361526798No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6300.1384402399630826No Hit
GTTCCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA6070.1333860724723669No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5900.12965038345749005No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5820.12789241215637157No Hit
CTTTTACACAATGTGGGTATCCTGCTTTAATGCCGTTGTATGCATGTATT5780.1270134265058123No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5510.12108027336453732No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5500.1208605269518975No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5360.11778407717494012No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5310.11668534511174106No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5280.1160261058738216No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5160.11338914892214384No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5150.11316940250950404No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT5140.11294965609686422No Hit
TCCTATCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATT5020.11031269914518645No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5010.11009295273254663No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5000.10987320631990682No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4960.10899422066934757No Hit
GGATACCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4940.10855472784406794No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT4900.1076757421935087No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4870.10701650295558926No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4870.10701650295558926No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4850.10657701013030962No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4710.10350056035335223No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4690.1030610675280726No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4670.10262157470279298No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4620.10152284263959391No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG4610.1013030962269541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)