FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621719
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA59480.9567023044172689No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20070.3228146477749594No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT19720.3171850948740508No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG17970.28903733036950774No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA17550.28228186688841744No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16760.26957516176922375No Hit
GCTTTATTCTTCTACTTTACCTGTCTTTAACCCTCATTGGAAAACACCCT16420.2641064532369125No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA16240.2612112546021595No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT15720.2528473474350953No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13850.22276945050738356No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG12530.20153799385252824No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC12430.19992955016655437No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT12410.19960786142935957No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC11610.18674031194156845No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11590.18641862320437366No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC11560.1859360900985815No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT11490.1848101795183998No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT11310.1819149808836468No Hit
GTTGTGGAGTTCCACTGCATGGCCTGAGGATGAGTGTCTCTCAAAGGTGG11250.18094991467206245No Hit
GCTGTATTCTTCTACTTTACCTGTCTTTAACCCTCATTGGAAAACACCCT11220.1804673815662703No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT10870.17483782866536168No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10360.16663476586689485No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG10140.1630961897577523No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10110.16261365665196012No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA9960.1602009911229993No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT9870.15875339180562278No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA9430.1516762395873377No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9300.14958526279557163No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC9240.1486201965839873No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC9090.14620753105502649No Hit
ACTTTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATAT9060.1457249979492343No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG9040.14540330921203953No Hit
ATCCAAAGGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT8910.14331233242027347No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8660.13929122320533874No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG8590.13816531262515702No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC8540.13736109078217007No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8520.1370394020449753No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8500.13671771330778054No Hit
GGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG8210.13205322661845625No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8120.13060562730107975No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8110.13044478293248238No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8040.12931887235230063No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA7820.12578029624315806No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA7710.1240110081885868No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAATGGGGGAAAGCCCTA7610.12240256450261291No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC7590.12208087576541815No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACGGT7490.12047243207944426No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7440.11966821023645731No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT7420.11934652149926253No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7410.11918567713066514No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT7340.11805976655048342No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7320.11773807781328864No Hit
TTGTATGCCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTA7180.1154862566529252No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCTAAGC7140.11484287917853564No Hit
AACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGA7130.11468203480993824No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT7100.1141995017041461No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7030.11307359112396435No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG6990.11243021364957481No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT6920.11130430306939308No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC6890.11082176996360092No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6810.1095350150148218No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAG6750.10856994880323748No Hit
GCAATGACCCATAACTTCCAATGACATAGCCCATGAAATTTAAAGAGTAA6670.10728319385445836No Hit
GACCTTTAACCTCGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC6670.10728319385445836No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGCCCACT6670.10728319385445836No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6610.10631812764287404No Hit
ACACTACTGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCT6550.1053530614312897No Hit
CACTACTGTGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6420.10326208463952363No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6410.10310124027092626No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6360.10229701842793931No Hit
TTGTAGGCCCACTCACGGTAAATGAGAAACGAAGACTGCAATTAATTATG6340.10197532969074452No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACGTAGCGCC6300.10133195221635498No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC6280.1010102634791602No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTGTTAACAGAC6270.1008494191105628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)