FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287574
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA20400.7093826284712804No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11400.39641970414571553No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9970.34669337283620916No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT8890.30913782191714134No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8210.285491734301432No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7190.2500226028778679No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT6700.2329835103312539No Hit
GTTAATATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTC6690.23263577374866992No Hit
ACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATAT6510.2263765152621586No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG6500.22602877867957463No Hit
TAACCACACTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6150.21385799828913601No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6030.2096851592981285No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5820.20238269106386528No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAATCAACACGTAGCGCC5470.19021191067342666No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5260.1829094424391635No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4970.17282508154422863No Hit
ATATAAGAGAGAATCAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4930.17143413521389278No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4750.1651748767273815No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4720.1641316669796296No Hit
GAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT4630.16100203773637395No Hit
ACTAAATGTTCCGGATAATAAGGTTTAATGCCCTTATCTAAGGGCAAATA4460.1550905158324466No Hit
CAGTAAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT4430.15404730608469472No Hit
ATCCAAATGTTACCAAATATTTGCCCTTAGATAAGGGCATTAAACCTTAT4290.14917899392851927No Hit
GTGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCTAAGC4280.1488312573459353No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTTTACCTGTC4230.14709257443301552No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4170.14500615493751173No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4170.14500615493751173No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC4160.14465841835492776No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4140.14396294518975986No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT4010.13944236961616835No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC3950.1373559501206646No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3910.13596500379032875No Hit
ATCTAAGGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAA3850.133878584294825No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC3840.13353084771224102No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3810.13248763796448915No Hit
ACTACATGTTCCGGATAATAAGGTTTAATGCCCTTATCTAAGGGCAAATA3750.13040121846898536No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3670.12761932580831367No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTCTCA3650.12692385264314576No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA3590.124837433147642No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3570.12414195998247407No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACGACAGAGT3540.12309875023472219No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3530.12275101365213824No Hit
GGATAATAAGGTTTAATGCCCTTATCTAAGGGCAAATATTTGGTAACATT3480.12101233073921842No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT3400.11823043807854673No Hit
GTAAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC3390.11788270149596279No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA3380.11753496491337882No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG3370.11718722833079485No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACACTGAGTAAAA3360.11683949174821089No Hit
ATTAATTGCAGTCTTCTTTTCTCATTTACTGTGAGTGGGCCTACAAATTG3360.11683949174821089No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3320.11544854541787507No Hit
CTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTA3270.11370986250495525No Hit
CTTTTACTCAGTGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT3210.11162344300945147No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT3210.11162344300945147No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3180.11058023326169959No Hit
ATGTTAATATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTAT3160.10988476009653168No Hit
TTCCCCCACTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3150.10953702351394771No Hit
TAATAAGGTTTAATGCCCTTATCTAAGGGCAAATATTTGGTAACATTTGG3150.10953702351394771No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC3100.1077983406010279No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA3080.10710286743585999No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3060.10640739427069205No Hit
AATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCCTTAG3050.10605965768810811No Hit
GCTTTATTCTTCTACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCT3050.10605965768810811No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTCTCATTTAC3040.10571192110552415No Hit
CCCTTATCTAAGGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG2980.10362550161002036No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2970.10327776502743642No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG2950.10258229186226848No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC2940.10223455527968454No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT2940.10223455527968454No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2920.10153908211451661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)