FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287574
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA19600.6815637018645636No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT10190.35434357765305624No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9970.34669337283620916No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT8420.29279420253569516No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8380.29140325620535934No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7520.26149791010313866No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG7240.25176128579078777No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT7230.2514135492082038No Hit
GTTAATATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTC7020.24411108097394063No Hit
TAACCACACTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6200.2155966812020558No Hit
ACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATAT5910.20551232030712094No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5870.20412137397678512No Hit
GAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5580.19403701308185023No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5440.18916870092567478No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAATCAACACGTAGCGCC5190.18047528636107577No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5020.17456376445714844No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4790.16656582305771733No Hit
ATATAAGAGAGAATCAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4580.15926335482345413No Hit
ACTAAATGTTCCGGATAATAAGGTTTAATGCCCTTATCTAAGGGCAAATA4490.1561337255801985No Hit
GTGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATTCAATCTAAGC4400.15300409633694284No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4360.151613150006607No Hit
CAGTAAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTAT4350.15126541342402303No Hit
ATCTAAGGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAA4320.15022220367627115No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4180.1453538915200957No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTTTACCTGTC4170.14500615493751173No Hit
ATCCAAATGTTACCAAATATTTGCCCTTAGATAAGGGCATTAAACCTTAT4110.14291973544200798No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA4090.14222426227684004No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTCTCA4000.1390946330335844No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT3980.13839915986841647No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA3920.13631274037291272No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT3870.1345740574599929No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3750.13040121846898536No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG3740.1300534818864014No Hit
ACTACATGTTCCGGATAATAAGGTTTAATGCCCTTATCTAAGGGCAAATA3720.1293580087212335No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACGACAGAGT3710.12901027213864952No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT3710.12901027213864952No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC3600.12518516973022595No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3600.12518516973022595No Hit
GTATGGATCGGCAGAGGAGCCAAAAAGGTTCCACGCATGCGCTGATGGCC3560.12379422339989012No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT3560.12379422339989012No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3550.12344648681730615No Hit
ATTAATTGCAGTCTTCTTTTCTCATTTACTGTGAGTGGGCCTACAAATTG3550.12344648681730615No Hit
GGATAATAAGGTTTAATGCCCTTATCTAAGGGCAAATATTTGGTAACATT3530.12275101365213824No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3490.12136006732180239No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3480.12101233073921842No Hit
CCCTTATCTAAGGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3480.12101233073921842No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA3470.12066459415663447No Hit
GGATAACCACACTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3390.11788270149596279No Hit
GGTTTGTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3350.11649175516562695No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC3310.1151008088352911No Hit
TAATAAGGTTTAATGCCCTTATCTAAGGGCAAATATTTGGTAACATTTGG3280.11405759908753922No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3240.11266665275720338No Hit
GTTTAATGCCCTTATCTAAGGGCAAATATTTGGTAACATTTGGATAAAAC3200.11127570642686753No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA3160.10988476009653168No Hit
ATGTTAATATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTAT3150.10953702351394771No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTCTCATTTAC3100.1077983406010279No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3090.10745060401844396No Hit
CCTCAATGTTAATATTCCTTGGACTCATAAAGTGGGTAACTTTACGGGGC3060.10640739427069205No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC3020.10501644794035621No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA3010.10466871135777227No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC2980.10362550161002036No Hit
GTATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT2960.10293002844485245No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA2950.10258229186226848No Hit
CCCCTACTCCGTCTGCCGTTTCGTCCGACCACGGGGCGCACCTCTCTTTA2930.10188681869710058No Hit
GGCATAATTAATTGCAGTCTTCTTTTCTCATTTACTGTGAGTGGGCCTAC2930.10188681869710058No Hit
GCTTTATTCTTCTACTTTACCTGTCTTTAATCCTCATTGGAAAACACCCT2920.10153908211451661No Hit
TTCCCCCACTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2910.10119134553193264No Hit
GTAAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC2910.10119134553193264No Hit
GGTTTAATGCCCTTATCTAAGGGCAAATATTTGGTAACATTTGGATAAAA2880.10014813578418076No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCCTTAGATAAGGGCATTAAAC2880.10014813578418076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)