FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422936
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29160.6894660184992528No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11220.2652883651427166No Hit
ATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10150.23998902907295666No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9950.2352601812094501No Hit
CTCTTTACACTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC8140.192464108044716No Hit
TGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATA8110.19175478086519002No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8100.1915183384720147No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8010.18939035693343673No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7920.1872623753948588No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGACAAAGACC7870.18608016342898218No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7870.18608016342898218No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCA7640.18064198838594966No Hit
TCCATAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA7500.1773317948814951No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7020.16598256000907938No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6570.15534265231618968No Hit
TTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC6490.15345111317078705No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT6440.15226890120491043No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6420.15179601641855978No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6290.14872226530728053No Hit
CTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGTTT6280.1484858229141052No Hit
CTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA6230.14730361094822858No Hit
ACACATAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCT6030.14257476308472203No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5980.1413925511188454No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCAACCAAACTCTGCAAG5740.13571793368263757No Hit
GTTTCCTAAAACATTCTTTGAGTTTCTGTATGATGTGATCTTGTGGCAAT5730.13548149128946224No Hit
GTGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5630.13311706735770898No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5610.1326441825713583No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCTACCAATCGCCAATC5570.131698412998657No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5420.1281517771010271No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACACTTTAT5400.12767889231467647No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5380.12720600752832578No Hit
GTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5160.12200427487846861No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5070.11987629333989067No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5060.11963985094671534No Hit
GGATAACCACATTGTGTGAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5010.1184576389808387No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG4970.11751186940813739No Hit
ATGTAAGACCTTGGGCAAGACTTGGTGGGCGTTCACGGTGGTCTCCATGC4960.11727542701496207No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT4900.11585677265591011No Hit
CTCCAAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA4840.11443811829685815No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC4730.11183725197192956No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4690.11089148239922825No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG4550.10758128889477368No Hit
CTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC4540.10734484650159834No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTATGTAAATAATGTCTGGT4540.10734484650159834No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG4520.1068719617152477No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACTTGGTGGGCGTTC4500.10639907692889705No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4480.10592619214254638No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCA4420.10450753778349442No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGAGTTTCTGT4350.10285244103126714No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4340.10261599863809182No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT4330.10237955624491649No Hit
TTATCTACATACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA4320.10214311385174117No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4320.10214311385174117No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4260.10072445949268921No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4240.10025157470633854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)