FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325677
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12940.39732618514663304No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATTGTCAGG9720.29845521789994384No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9600.2947705855801914No Hit
CTCTGATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8420.2585383677692929No Hit
GTTTAATGCCTTTATCCAAGGGGAAATATTTGGTAAGGCTAGGATAGAAC7980.24502804926353414No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCAAAG7770.2385799427039674No Hit
GGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCT7450.22875425651796105No Hit
GTATTTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTT7440.22844720382464834No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7400.22721899305139753No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAA7060.21677920147876575No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7020.21555099070551498No Hit
ATCCTAGCCTTACCAAATATTTCCCCTTGGATAAAGGCATTAAACCGTAT6850.21033109491919907No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6710.20603235721282132No Hit
GTTTTATGCCTTTATCCAAGGGGAAATATTTGGTAAGGCTAGGATAGAAC6130.1882233010006847No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6010.18453866868093233No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCG5980.18361751060099424No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5830.17901172020130376No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5200.15966740052260367No Hit
GGCTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5060.15536866281622588No Hit
TATGCGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCT4920.1510699251098481No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA4900.15045581972322272No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4800.1473852927900957No Hit
GCATTAAACCGTATTATCCTGACAATGTAGTTAATCATTACTTCAAAACT4690.14400771316365601No Hit
GCCTAGATTGTATGCATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4690.14400771316365601No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA4530.13909487007065283No Hit
CTATTATGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4510.13848076468402742No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4480.13755960660408934No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC4470.13725255391077662No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4430.13602434313752584No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGGAAATATTTGGTAAGGCT4360.13387497428433692No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4350.13356792159102424No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4320.13264676351108615No Hit
ATATCAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4280.13141855273783534No Hit
GTTCTATCCTAGCCTTACCAAATATTTCCCCTTGGATAAAGGCATTAAAC4280.13141855273783534No Hit
CCTCCCCCGTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA4260.13080444735120994No Hit
CAATATTAATATGGGCCTGAAAATCAGACAACTATTATGGTTTCACATTT4170.12804097311139565No Hit
GTATTATCCTGACAATGTAGTTAATCATTACTTCAAAACTAGGCATTATT4090.12558455156489404No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT4070.12497044617826865No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4060.12466339348495596No Hit
ATATAAGAGAGAAACTACACACAGCGCCTCATTTTGTGGGTCACCATATT3950.12128581385851626No Hit
TGTCAGGATAATACGGTTTAATGCCTTTATCCAAGGGGAAATATTTGGTA3920.12036465577857816No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAAATTCATTTACAGG3910.12005760308526547No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3700.11360949652569877No Hit
AATACGGTTTAATGCCTTTATCCAAGGGGAAATATTTGGTAAGGCTAGGA3660.11238128575244796No Hit
CTTTAGGACATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTTGA3540.10869665343269559No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3420.1050120211129432No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC3410.10470496841963048No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3320.1019414941798162No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3320.1019414941798162No Hit
ATATAGAGCTGTGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT3310.10163444148650351No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT3310.10163444148650351No Hit
GCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACA3280.1007132834065654No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3270.1004062307132527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)