FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428230
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT16210.37853489946991103No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15470.3612544660579595No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACA13380.31244891763771804No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCAAGGACAAAGACC12930.30194054596828807No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA12610.2944679261144712No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10990.25663778810452326No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10690.24963220699156996No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10640.24846461013941107No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10620.2479975713985475No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10530.2458958970646615No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATATTCAA9600.2241785956145062No Hit
CATGTATACAAGCCAAACAAGCTTTTACTTTCTCGCCAACTTACAAGGCC9350.21834061135371177No Hit
CTCCAAAAGACACTAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8260.19288699997664807No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8190.1912523643836256No Hit
ACTACATGCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA8000.18681549634542186No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATCTTTTGTACAATATGTT7900.18448030264110407No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGA7610.17770824089858253No Hit
AGTCTGTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTT7580.1770076827872872No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT7510.17537304719426478No Hit
GGAACATATTGTACAAAAGATCAAAATGTGTTTTAGGAAACTTCCTGTAA7440.17373841160124232No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7420.17327137286037878No Hit
CATTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7250.16930154356303856No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7230.168834504822175No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGAAACCCGTG7220.16860098545174323No Hit
GAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT6890.1608948462274946No Hit
GTATTATCCAGAGCATGTAGTTGATCATTACTTCCAGACGCGACATTATT6820.15926021063447213No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATCTTTTGT6800.15879317189360856No Hit
GATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGAGACTA6620.1545898232258366No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6590.15388926511454126No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6520.1522546295215188No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6410.14968591644676926No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6340.1480512808537468No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACATGCTCTGGAT6110.14268033533381594No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT6100.14244681596338415No Hit
CCCCACGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCAC6050.14127921911122526No Hit
TCCTCAGGCCATGCAGTGGAATTCCACCACTTTCCACCAAACTCTTCAAG5920.13824346729561218No Hit
CAGTAAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCG5920.13824346729561218No Hit
ATCCCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5880.13730938981388505No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5830.13614179296172618No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC5800.13544123485043086No Hit
CCATTCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAG5620.13123788618265886No Hit
GGTTTCCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG5540.12936973121920464No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5480.12796861499661397No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5470.12773509562618218No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5440.12703453751488686No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCGTG5430.12680101814445507No Hit
TGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATG5350.12493286318100087No Hit
CCAAAAGACACTAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5320.12423230506970553No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5260.12283118884711486No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5250.12259766947668307No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAA5240.12236415010625132No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5170.12072951451322887No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5160.12049599514279709No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5150.12026247577236532No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAATTCCACCACTTT5130.11979543703150175No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5050.11792728206804755No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAATCTAGCAGGC5030.117460243327184No Hit
CTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATATTC5020.11722672395675221No Hit
GTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAG5010.11699320458632044No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5000.11675968521588866No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4990.11652616584545687No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG4920.11489153025243445No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4900.11442449151157089No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC4870.11372393340027555No Hit
GTACAATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC4850.113256894659412No Hit
CCTCCATATAGACCGCCAAATGCCCCTATCTTATCAACACTTCCGGAAAC4850.113256894659412No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4800.1120892978072531No Hit
GTACAACATCTTGAGTCCATTTATACCGCTGTTACCAATTTTCTTTTGTC4790.11185577843682135No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATGTGGTTTTGGGGGC4780.11162225906638956No Hit
GTACTGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT4730.11045466221423067No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA4700.10975410410293533No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4680.10928706536207178No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4660.10882002662120822No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4650.10858650725077644No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCATAAAACTCAGA4640.10835298788034467No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAATCTAGCAGGCATAA4620.10788594913948113No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4580.10695187165775401No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4560.10648483291689045No Hit
ATATTGTACAAAAGATCAAAATGTGTTTTAGGAAACTTCCTGTAAACAGG4540.1060177941760269No Hit
GTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATAT4540.1060177941760269No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4520.10555075543516335No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4510.10531723606473156No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4500.10508371669429978No Hit
ATCCAGAGCATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACAC4480.10461667795343625No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC4470.10438315858300447No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4470.10438315858300447No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4460.10414963921257268No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAAC4390.10251500361955024No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACATGCTCTGG4380.10228148424911845No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4320.1008803680265278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)