FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560252
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31290.5584986755959819No Hit
AGTCTAGACTCTGTGGTATTGTGAGGACTTTTGTCAACAAGAAAAACCCC14080.2513154794628132No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13770.24578225512804952No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11780.21026252472101842No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAAACACCCGTG11370.20294438931052458No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAATGTGTAAATAATGTCGCGT10790.19259190507128934No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10650.19009302956526705No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10250.18295338526234622No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9450.16867409665650457No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9250.16510427450504417No Hit
GCACCGGACCGTGCAAGACCTGCACAACTCCTGCTCAAGGAACCTCTATG8990.16046350570814563No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT8580.15314537029765177No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC8500.1517174414370676No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8290.14796912817803418No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7690.13725966172365292No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7670.1369026795085069No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG7570.13511776843277667No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7390.13190492849646232No Hit
CTCCAAAAGACACCAAATATTCAAGAACAATTTGTCTCCCAAAAGTAAGA7180.12815661523742888No Hit
GTATAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC6680.11923205985877783No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATACTCCCTTAACTTCAT6680.11923205985877783No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6630.11833960432091273No Hit
ACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACAT6630.11833960432091273No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6630.11833960432091273No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6610.11798262210576668No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6540.11673318435275555No Hit
CGTTAATATGGGCCTAAAAATCAGGCAACTATTGTGGTTTCACATTTCCT6530.11655469324518253No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAAGCC6370.1136988355240142No Hit
ATATGGGGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6360.11352034441644117No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6300.11244939777100305No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6260.11173543334071097No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6190.11048599558769981No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6180.1103075044801268No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6070.10834410229682356No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5940.10602371789837431No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5930.10584522679080129No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5920.10566673568322825No Hit
CTCTTTTATATAAGATTCCCGCCTTCCAAAGAATGTGTAAATAATGTCGC5910.10548824457565524No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5910.10548824457565524No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5910.10548824457565524No Hit
CTGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC5890.10513126236050921No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5670.10120445799390275No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5620.10031200245603765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)