FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560252
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31140.5558213089823865No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13680.24417583515989233No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAAACACCCGTG12550.22400634000414096No Hit
AGTCTAGACTCTGTGGTATTGTGAGGACTTTTGTCAACAAGAAAAACCCC12310.2197225534223885No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11930.21293989133461372No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAATGTGTAAATAATGTCGCGT10860.1938413428243005No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10030.17902658089573978No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9430.16831711444135852No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9120.1627838901065949No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT9040.16135596124601073No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8690.155108772480955No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC8070.1440423238114277No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8000.14279288605841658No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7930.1415434483054054No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7750.13833060836909106No Hit
GCACCGGACCGTGCAAGACCTGCACAACTCCTGCTCAAGGAACCTCTATG7690.13725966172365292No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7360.13136945517374324No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7280.12994152631315906No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7090.1265501952692717No Hit
CTCCAAAAGACACCAAATATTCAAGAACAATTTGTCTCCCAAAAGTAAGA6980.12458679308596846No Hit
ATATGGGGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6620.1181611132133397No Hit
ACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACAT6550.11691167546032857No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATACTCCCTTAACTTCAT6490.11584072881489045No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6470.1154837465997444No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6360.11352034441644117No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6340.11316336220129512No Hit
GTATAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC6320.11280637998614909No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6190.11048599558769981No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAAGCC6130.10941504894226169No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6060.10816561118925055No Hit
CTCTTTTATATAAGATTCCCGCCTTCCAAAGAATGTGTAAATAATGTCGC5950.10620220900594733No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5950.10620220900594733No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG5930.10584522679080129No Hit
CTGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC5850.10441729793021712No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAGCATTGTACACCTCACCATACGG5830.10406031571507109No Hit
CGTTAATATGGGCCTAAAAATCAGGCAACTATTGTGGTTTCACATTTCCT5760.10281087796205993No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5720.10209691353176785No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5670.10120445799390275No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5620.10031200245603765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)