FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358598
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTGGTTT18330.5111573405317374No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCATAT16590.4626350397938639No Hit
GTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG12920.36029202616857875No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12000.3346365568129214No Hit
CCCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCAAGG11260.314000635809458No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10380.28946062164317704No Hit
GGTTAGGATAGAACCTTGCAGGCATGATTAATTTTAATCTCCTTTTTTCA9820.2738442489919074No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTGGT9400.26213196950345513No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8730.24344809508140036No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC8510.23731309153983013No Hit
GGATAATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT8470.2361976363504537No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC8050.22448535686200147No Hit
GTATACATGCGTATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7430.20719580142666716No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA7340.20468602725057025No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7180.20022420649306463No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6950.19381033915415033No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA6650.1854444252338273No Hit
GGATAGAACCTTGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC6550.18265578726038628No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6550.18265578726038628No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTCTGACATACT6380.17791510270553657No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6120.1706646439745899No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6120.1706646439745899No Hit
CTGTAAGGGGGCCCACATATTGTTGACATCTATCAACAATGTCCTCCTGT6030.16815486979849303No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6030.16815486979849303No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5790.1614621386622346No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTTGCAGGCATGA5790.1614621386622346No Hit
GAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTGCATGTTCA5650.15755804549941718No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5610.15644259031004076No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGACATTATT5530.15421167993128795No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT5500.15337508853925566No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5480.15281736094456747No Hit
CTGACATACTTTCCAATCAATGGGTCTATTTACAGGCAGTTTTCGAAAAC5410.15086531436315875No Hit
CATTTGAACCCTAATAAAACCAAGAGGTGGGGCTACTCCCTTAACTTCAT5360.14947099537643824No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5310.14807667638971775No Hit
TCCACAGAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTGCA5290.14751894879502955No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCCACTTACAAGGCC5270.14696122120034133No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA5250.14640349360565313No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC5150.1436148556322121No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5100.1422205366454916No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA5100.1422205366454916No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT4940.137758715887986No Hit
AAAACTCAGACAACTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA4940.137758715887986No Hit
CATTTACAGGAGGACATTGTTGATAGATGTCAACAATATGTGGGCCCCCT4910.1369221244959537No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4910.1369221244959537No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4800.13385462272516857No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4790.13357575892782447No Hit
GTTGATAGATGTCAACAATATGTGGGCCCCCTTACAGTTAATGAAAAAAG4660.12995052956235117No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4630.12911393817031885No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4630.12911393817031885No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4620.12883507437297476No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4610.12855621057563066No Hit
CCCTTAGACAAAGGCATTAAACCATATTATCCTGAACATGCAGTTAATCA4590.12799848298094246No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4580.12771961918359834No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA4570.12744075538625424No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4500.12548870880484553No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTGGGCGA4480.12493098121015733No Hit
AGTTAAGGGAGTAGCCCCACCTCTTGGTTTTATTAGGGTTCAAATGTATA4370.12186347943937222No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4280.11935370526327531No Hit
CTTCCAAACCTCGACAAGGCATGGGGACAAATCTTTCTGTTCCCAATCCT4230.1179593862765548No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4220.1176805224792107No Hit
GATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTGTCTAAGGGC4140.1154496121004579No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCACCTCTTGGTTTTA4110.11461302070842559No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATACGCATGTATA4070.11349756551904917No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4030.11238211032967278No Hit
ATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT4010.11182438273498459No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGACATTATTTACAT3980.11098779134295228No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3970.11070892754560817No Hit
ATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGAT3970.11070892754560817No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC3910.10903574476154357No Hit
GGATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3890.10847801716685536No Hit
CAATATGTGGGCCCCCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT3810.10624710678810256No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA3760.10485278780138206No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3730.10401619640934975No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3700.10317960501731743No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3650.10178528603059693No Hit
TCTATCAACAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTT3610.10066983084122054No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3590.10011210324653232No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC3590.10011210324653232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)