FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358598
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTGGTTT18180.506974383571576No Hit
GTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG16610.46319276738855214No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCATAT13360.3725620332517192No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11910.33212678263682455No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTGGT10430.2908549406298976No Hit
GGTTAGGATAGAACCTTGCAGGCATGATTAATTTTAATCTCCTTTTTTCA10090.2813735715201981No Hit
CCCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCAAGG9780.272728793802531No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC9720.27105561101846637No Hit
GGATAATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT8810.24567900546015314No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8800.24540014166280905No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC8500.237034227742486No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC8070.2250430844566897No Hit
GTATACATGCGTATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7530.2099844394001082No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7450.20775352902135538No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA7440.20747466522401128No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA7140.19910875130368824No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTCTGACATACT7070.19715670472227956No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7040.19632011333024724No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6650.1854444252338273No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTTGCAGGCATGA6470.18042487688163347No Hit
GAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTGCATGTTCA6420.17903055789491296No Hit
GGATAGAACCTTGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC6380.17791510270553657No Hit
CTGTAAGGGGGCCCACATATTGTTGACATCTATCAACAATGTCCTCCTGT5990.16703941460911662No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA5880.1639719128383315No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5820.1622987300542669No Hit
TCCACAGAGTATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTGCA5730.15978895587817No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA5680.15839463689144948No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5610.15644259031004076No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5410.15086531436315875No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5290.14751894879502955No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5240.14612462980830904No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT5240.14612462980830904No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC5220.1455669022136208No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGACATTATT5140.14333599183486803No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC5030.1402684900640829No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4990.1391530348747065No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTGGGCGA4980.13887417107736238No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4970.1385953072800183No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4960.1383164434826742No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT4930.1374798520906419No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT4900.13664326069860958No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4810.13413348652251267No Hit
CATTTGAACCCTAATAAAACCAAGAGGTGGGGCTACTCCCTTAACTTCAT4760.13273916753579218No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4670.13022939335969524No Hit
ATATGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGAT4670.13022939335969524No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4600.12827734677828656No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATACGCATGTATA4540.12660416399422195No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4390.12242120703406044No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4360.12158461564202813No Hit
CTGACATACTTTCCAATCAATGGGTCTATTTACAGGCAGTTTTCGAAAAC4300.11991143285796352No Hit
AAAACTCAGACAACTACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA4300.11991143285796352No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4270.1190748414659312No Hit
ATGTAAATAATGTCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT4260.11879597766858711No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4250.118517113871243No Hit
AGTTAAGGGAGTAGCCCCACCTCTTGGTTTTATTAGGGTTCAAATGTATA4220.1176805224792107No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4080.11377642931639328No Hit
GTTGATAGATGTCAACAATATGTGGGCCCCCTTACAGTTAATGAAAAAAG4020.11210324653232867No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG4000.11154551893764048No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4000.11154551893764048No Hit
CCCTTAGACAAAGGCATTAAACCATATTATCCTGAACATGCAGTTAATCA3980.11098779134295228No Hit
CATTTACAGGAGGACATTGTTGATAGATGTCAACAATATGTGGGCCCCCT3970.11070892754560817No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3880.10819915336951126No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3840.10708369818013484No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC3840.10708369818013484No Hit
GATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTGTCTAAGGGC3800.10596824299075845No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCCACTTACAAGGCC3760.10485278780138206No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC3720.10373733261200564No Hit
CTTCCAAACCTCGACAAGGCATGGGGACAAATCTTTCTGTTCCCAATCCT3700.10317960501731743No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3690.10290074121997334No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCACCTCTTGGTTTTA3670.10234301362528513No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3660.10206414982794104No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3650.10178528603059693No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3650.10178528603059693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)