FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438167
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT17460.39847820579824583No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17200.39254439517352974No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16060.3665269178190051No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAAGGCAAATATTTAGTAACATT15100.3446174632046686No Hit
CCTCCTGCATATAGACCTCAAAATGCCCCTATCTTATCAACACTTCCGGA13150.3001138835192974No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11940.27249884176581074No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11910.27181417130911273No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA11670.26633680765552864No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG10580.241460447728834No Hit
CCCTTACAGTAAATGAAAAAAGAAGACTAAAATTAATTATGCCTGCTAGG10470.23894998938760792No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT9240.21087850066298922No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC9090.20745514837949916No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8950.20426001958157505No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8910.20334712563931107No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTTAT8900.20311890215374503No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7850.17915543616931442No Hit
ATACATAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT7800.1780143187414844No Hit
GTTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG7770.1773296482847864No Hit
ACATAGAGGTGCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG7690.17550386040025834No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA7680.17527563691469233No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7630.17413451948686232No Hit
ATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGGAGGAC7560.1725369550879003No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCACCTCTATG7460.1702547202322402No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7090.161810451266298No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7080.161582227780732No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7060.1611257808096No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6830.15587664064158185No Hit
GCCTAGCAGCCATGGAAATGATGTATACCGGCGGGAGAGGACGACAGAAT6660.15199684138695976No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6600.15062750047356374No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6390.1458348072766776No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC6380.1456065837911116No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6350.1449219133344136No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6350.1449219133344136No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6280.14332434893545154No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT6260.14286790196431953No Hit
CTGTAAGGGGCCCCACAAATTGATTACATCTATCAACAATGTCCTCCTGC6140.14012922013752746No Hit
CAATTACATATCCCATAAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5780.13191317465715127No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTCTGGA5760.13145672768601926No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC5760.13145672768601926No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG5690.1298591632870572No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT5660.1291744928303592No Hit
CTCCAAAAGACACCAAATATTCAAGAACAATGTCTCTCCCAAAAGTAAGA5660.1291744928303592No Hit
CTCTAGAGCCTCCCGATACATAGCAGAGGCGGTGTCGAGGAGATCTCGAA5650.1289462693447932No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCTTTAGATAAAGGGATCAAAC5570.12712048146026517No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5540.12643581100356713No Hit
CGTTAATATGGGCCTAAAACTTAGACAACTATTGTGGTTTCACATTTCCT5520.12597936403243512No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5520.12597936403243512No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5500.1255229170613031No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5490.1252946935757371No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5490.1252946935757371No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC5480.1250664700901711No Hit
GATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAGAAGACTA5470.12483824660460509No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5390.12301245872007704No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5380.12278423523451104No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACACCCAATTACATATCCCATAAA5350.12209956477781303No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAAGGCAAATA5350.12209956477781303No Hit
ACTTGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5340.12187134129224703No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5330.12164311780668102No Hit
CCAAAAGACACCAAATATTCAAGAACAATGTCTCTCCCAAAAGTAAGACA5320.121414894321115No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5290.120730223864417No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAGGT5260.12004555340771898No Hit
GCTTTATTCTTCTACGGTACCTTGCTTCAATCCTAAATGGCAAACTCCTT5250.11981732992215298No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5200.11867621249432295No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5170.11799154203762491No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5140.11730687158092691No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5060.11548108369639887No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5050.11525286021083286No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5030.11479641323970086No Hit
GTTGCAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5010.11433996626856882No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4960.11319884884073882No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4950.11297062535517281No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA4880.11137306095621077No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4860.11091661398507874No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG4850.11068839049951273No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4840.11046016701394673No Hit
GCTCTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4820.11000372004281472No Hit
ATCCCAATGTTACTAAATATTTGCCTTTAGATAAAGGGATCAAACCTTAT4690.10703681473045665No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4680.10680859124489064No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4620.10543925033149461No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4600.1049828033603626No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4570.10429813290366459No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4570.10429813290366459No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG4570.10429813290366459No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACGT4520.10315701547583456No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4490.10247234501913655No Hit
GGGTAAAGGTTCAGATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGA4480.10224412153357053No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4470.10201589804800452No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4470.10201589804800452No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4460.10178767456243852No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4450.10155945107687252No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAAGGCAAATATTTAGTA4440.10133122759130651No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT4420.1008747806201745No Hit
GCTATGTATCGGGAGGCTCTAGAGTCTCCGGAACATTGTTCACCTCACCA4390.10019011016347648No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4390.10019011016347648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)