FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438167
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT16820.38387190272202154No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15590.3558004139974028No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAAGGCAAATATTTAGTAACATT15290.34895370943042264No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15120.3450739101758006No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12120.27660686450599886No Hit
CCTCCTGCATATAGACCTCAAAATGCCCCTATCTTATCAACACTTCCGGA12120.27660686450599886No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG11550.26359812582873654No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10840.24739425835355014No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT10160.23187506133506172No Hit
CCCTTACAGTAAATGAAAAAAGAAGACTAAAATTAATTATGCCTGCTAGG9790.22343079236911953No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9760.22274612191242155No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC9040.20631403095166911No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8850.201977784725915No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTTAT8350.19056661044761472No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8300.18942549301978467No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7760.1771014247992204No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7300.1666031444631841No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCACCTCTATG7290.1663749209776181No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7100.162038674751864No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7060.1611257808096No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7050.160897557324034No Hit
ATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGGAGGAC7040.160669333838468No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6850.15633308761271386No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA6810.15542019367044985No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCTTTAGATAAAGGGATCAAAC6710.1531379588147898No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6660.15199684138695976No Hit
GTTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG6580.15017105350243173No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6510.14857348910346968No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG6330.14446546636328159No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6250.14263967847875356No Hit
ACATAGAGGTGCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG6190.1412703375653575No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC6100.13921632619526345No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5980.13647764436847137No Hit
CTCCAAAAGACACCAAATATTCAAGAACAATGTCTCTCCCAAAAGTAAGA5900.13465185648394334No Hit
CTGTAAGGGGCCCCACAAATTGATTACATCTATCAACAATGTCCTCCTGC5870.13396718602724533No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT5660.1291744928303592No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5620.12826159888809516No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5500.1255229170613031No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5490.1252946935757371No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5420.12369712917677507No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5270.12027377689328499No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC5230.11936088295102096No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5210.11890443597988895No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT5190.11844798900875694No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACACCCAATTACATATCCCATAAA5170.11799154203762491No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5130.11707864809536091No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5050.11525286021083286No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAAGGCAAATA5030.11479641323970086No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAGGT5020.11456818975413485No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5000.11411174278300282No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4960.11319884884073882No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTCTGGA4930.11251417838404078No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4900.11182950792734277No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCTG4880.11137306095621077No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4880.11137306095621077No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAAGGCAAATATTTAGTA4850.11068839049951273No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4840.11046016701394673No Hit
CGTTAATATGGGCCTAAAACTTAGACAACTATTGTGGTTTCACATTTCCT4830.11023194352838073No Hit
CAATTACATATCCCATAAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4750.10840615564385268No Hit
GCTCTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4720.10772148518715467No Hit
GCTTTATTCTTCTACGGTACCTTGCTTCAATCCTAAATGGCAAACTCCTT4720.10772148518715467No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4670.10658036775932464No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4670.10658036775932464No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4670.10658036775932464No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4650.10612392078819263No Hit
GTTGCAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4630.10566747381706061No Hit
GATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAGAAGACTA4610.1052110268459286No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4590.1047545798747966No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4540.10361346244696656No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4520.10315701547583456No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4500.10270056850470255No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4480.10224412153357053No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTTTTTTCATTTAC4440.10133122759130651No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4410.1006465571346085No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4410.1006465571346085No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4390.10019011016347648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)