FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628149
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41450.6598752843672441No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA22700.3613792269031711No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT20300.32317173154776974No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15710.25009989668056465No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC15050.23959283545782928No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14500.2308369511055498No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13080.20823084968693734No Hit
GCACTGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG12810.2039325064594547No Hit
GTCCAGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC12430.19788298636151613No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG12380.19708699687494527No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGG12300.1958134136964319No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10420.16588420900136752No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9930.15808351203297305No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9600.1528299814216054No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9560.1521931898323487No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9260.1474172529129235No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA9220.14678046132366684No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG9110.14502928445321092No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8380.13340783794927635No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC8360.13308944215464802No Hit
CGTTAATATGGGCCTAAAACTTAGACAACTATTGTGGTTTCACATTTCCT8290.1319750568734488No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8130.12942789051642206No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8030.12783591154328033No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT7950.12656232836476697No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7740.12321917252116933No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7640.12162719354802762No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC7520.11971681878025756No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7440.11844323560174416No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACGGAAT7440.11844323560174416No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7090.11287130919574814No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7050.11223451760649145No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAGCACAGTTTGATTTTTTGT6870.10936895545483635No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6840.10889136176289384No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6730.10714018489243794No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG6690.10650339330318126No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6590.10491141433003953No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTACGG6580.10475221643272536No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTA6570.10459301853541118No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6570.10459301853541118No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6470.10300103956226946No Hit
GGAACATATTGTACAAAAAATCAAACTGTGCTTTAGAAAACTTCCTGTAA6440.10252344587032694No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6410.10204585217838444No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6370.10140906058912773No Hit
GGGAATCAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTTAGA6310.10045387320524271No Hit
GTTTACAGGAAGTTTTCTAAAGCACAGTTTGATTTTTTGTACAATATGTT6290.10013547741061436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)