FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628149
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41210.656054534831704No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA21330.33956911497112946No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT19740.3142566492981761No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15120.24070722073902848No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG14550.23163294059212067No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14070.2239914415210404No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC12990.20679806861110978No Hit
GTCCAGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC12640.20122614220511376No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12270.1953358200044894No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAGTTATGCCTGCTAGG12030.19151507046894925No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11770.18737592513878076No Hit
GCACTGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG11560.18403276929518314No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10710.1705009480234785No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT10500.1671577921798809No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG9580.15251158562697703No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9140.14550687814515348No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8910.1418453265069275No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA8170.13006468210567876No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8010.12751751574865197No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT7990.12719911995402364No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC7890.1256071409808819No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC7760.1235375683157977No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7760.1235375683157977No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7640.12162719354802762No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7610.12114959985608509No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7490.11923922508831504No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7420.11812483980711583No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7280.11589606924471742No Hit
CGTTAATATGGGCCTAAAACTTAGACAACTATTGTGGTTTCACATTTCCT7210.11478168396351822No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG7170.11414489237426151No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC7130.11350810078500484No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7130.11350810078500484No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6910.11000574704409304No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6900.10984654914677887No Hit
GTTTACAGGAAGTTTTCTAAAGCACAGTTTGATTTTTTGTACAATATGTT6690.10650339330318126No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6640.10570740381661038No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6610.10522981012466787No Hit
GGAACATATTGTACAAAAAATCAAACTGTGCTTTAGAAAACTTCCTGTAA6580.10475221643272536No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT6470.10300103956226946No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACGGAAT6400.10188665428107026No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG6330.10077226899987105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)