FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410298
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG18880.46015335195394563No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18000.43870552622727865No Hit
ATCCTAACCTTACCAAATATTTGCCTTTGGACAAAGGCATTAAACCGTAT15960.3889855665881871No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCAGCTTTCCACAGAGTATGTAAA12680.30904367069788297No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11640.2836962402936402No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTGTAATCTCCTTTTTTCA9990.24348156705613966No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTACAATTAATTATGCCTGCTAGG9370.22837059893053344No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9070.22105884016007876No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACAGACT8900.21691551019015448No Hit
CTTATATAAAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTGGTTT8430.20546042144977553No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTGTCTTCCAAAAGAAAGG7880.19205553037060868No Hit
ATCCTAAACTTACCAAATATTTGCCTTTGGACAAAGGCATTAAACCGTAT7730.18839965098538136No Hit
CATTTAAACCCTAACAAAACTAAGAGATGGGGTTATTCTCTAAATTTCAT7720.18815592569303285No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7000.17060770464394173No Hit
CTTTTACACAATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATT5990.14599145011674441No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATGA5670.13819224076159278No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT5670.13819224076159278No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC5670.13819224076159278No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG5630.13721733959219884No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA5610.13672988900750185No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5540.13502381196106245No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT5410.13185538316053208No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5180.12624970143651687No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5140.12527480026712293No Hit
ATGTTCGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGC5130.12503107497477442No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC4980.12137519558954712No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACCACCGTG4960.12088774500485014No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4930.12015656912780466No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGCGAGAACAACAGAGT4900.11942539325075921No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC4900.11942539325075921No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4830.11771931620431979No Hit
GAATACATGCATACAAGGGCATTAAGGCAGGATATCCACATTGTGTAAAA4820.11747559091197131No Hit
CTCTTATATAAAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTGGT4820.11747559091197131No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAAGGCAAATATTTGGTA4730.1152820632808349No Hit
AAGTCGGTGAGAATGTGAAAGCCTGTTTGGATTGAATACATGCATACAAG4680.11406343681909245No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4640.11308853564969851No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTGAGGAAAGGATGGAGTTTG4590.11186990918795607No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4590.11186990918795607No Hit
GTCCTATTGATTGGAAAGTCTGTCAACGAATTGTGGGTCTTTTGGGTTTT4560.11113873331091059No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTAAAAG4560.11113873331091059No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4450.10845775509507724No Hit
ATGTAACACCTTGGGCAACACCAGGTGGGCGTTCACGGTGGTCTCCATGC4440.10821402980272873No Hit
GTGTCAAGGAGATCCCGAATAGAAGGAAAGAAGTCATTAGGCAAAAAAGA4420.10772657921803176No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4410.10748285392568328No Hit
ATGTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATTCAATCCAAAC4410.10748285392568328No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATTTACAT4370.10650795275628934No Hit
GTTTCCTAAAACAATCTTTGATTTTTTGTATGATGTGTGATTGTGGCAAG4310.10504560100219841No Hit
CTACTGTACCTGTCTTTAACCCGGAGTGGCAAACTCCATCCTTTCCTCAC4290.10455815041750142No Hit
GTGGATATCCTGCCTTAATGCCCTTGTATGCATGTATTCAATCCAAACAG4240.10333952395575897No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4190.10212089749401655No Hit
CTCAAGAACAGTTTGTCTTCCAAAAGAAAGGCAGGAAATGTGAAACCACA4170.10163344690931958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)