FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366068
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGG26600.7266409519542817No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG18910.5165706917840401No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15070.4116721483440235No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT13320.36386682255755765No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12800.34966181146672204No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11180.30540773845296504No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT10940.298851579487964No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG10530.28765147458942053No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCTC10440.2851929149775452No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT9560.26115366543920804No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT8710.237933935771496No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8680.2371144159008709No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8560.23383633641837037No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA8420.2300119103554531No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8360.2283728706142028No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8210.22427527126107719No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7950.21717276571565938No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7770.21225564649190862No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7760.21198247320170024No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCA7620.20815804713878297No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7560.20651900739753268No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT7450.20351410120524055No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTC7390.20187506146399029No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7340.20050919501294842No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7170.19586524907940603No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT7070.19313351617732225No Hit
GATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC7030.19204082301648873No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT7020.19176764972628038No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6960.19012860998503012No Hit
ATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6720.18357245102002906No Hit
CTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCTCATTCATTTGAAAG6580.17974802495711178No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6420.17537725231377777No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6320.172645519411694No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6290.17182599954106886No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA6090.16636253373690132No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6010.16417714741523431No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCT5930.1619917610935673No Hit
GTTTGGAAGTAATGATTAACTGTGTGTTCAGGATAATAAGGTTTAATGCC5880.16062589464252544No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT5780.15789416174044166No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTG5700.15570877541877465No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5560.15188434935585737No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5540.15133800277544063No Hit
ATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA5470.149425789743982No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5370.14669405684189823No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5350.14614771026148146No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5310.14505501710064797No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT5260.14368915064960608No Hit
GAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTTCCTGTGGT5260.14368915064960608No Hit
GGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAA5230.14286963077898096No Hit
CCTCTCATTCATTTGAAAGAGGATATTATCAATAGATGTCAACAATATGT5230.14286963077898096No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACTCACG5170.1412305910377307No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5050.1379525115552302No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA5040.1376793382650218No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5040.1376793382650218No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5010.13685981839439668No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAAC4980.13604029852377153No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA4920.1344012587825213No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT4890.13358173891189615No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA4810.13139635259022914No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA4700.128391446397937No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4680.12784509981752024No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGAGAGG4640.12675240665668674No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4540.12402067375460296No Hit
ACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA4530.12374750046439459No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4520.12347432717418623No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4450.12156211414272758No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA4440.12128894085251919No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4290.11719134149939356No Hit
CTCCCATAGGAATCTTACGAAAGCCCAAGATGATGGGATGGGAATACAAG4280.11691816820918519No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA4250.11609864833856005No Hit
GCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAG4200.11473278188751818No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4190.11445960859730979No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4140.1130937421462679No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4140.1130937421462679No Hit
AACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAAT4140.1130937421462679No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4110.11227422227564278No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4090.11172787569522602No Hit
GTTTCTCTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATG4090.11172787569522602No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCAGGAT4070.11118152911480927No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA4070.11118152911480927No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4040.11036200924418413No Hit
ATTTTAATCTCCTTTTTTCGTTAACTGTAAGAGGGCCTACATATTGTTGA4040.11036200924418413No Hit
ACCCAGCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGC4010.10954248937355901No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA3950.10790344963230875No Hit
GTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGG3930.107357103051892No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3880.10599123660085012No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3840.10489854344001662No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3830.10462537014980823No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGT3830.10462537014980823No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA3780.10325950369876634No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3760.10271315711834961No Hit
CTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCT3670.10025459750647421No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3670.10025459750647421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)