FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463377
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98432.1241882959231897No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12810.27644876633928744No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12570.27126939835166614No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAC11060.23868254142954873No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10930.23587705043625384No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10270.22163378847029525No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10230.22077056047235835No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9970.21515957848576864No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9320.20113212351929424No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8600.1855940195564303No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7930.1711349505909875No Hit
GCCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7880.17005591559356636No Hit
ATCCTAACCTTACTAAGTATTTGCCCTTAGACAAAGGCATTAAACCGTAT7670.16552396860439772No Hit
GTGTAAACGGGGCAGCAAAGCCTAAAAGACCCACAATTCTTTGACATACT6990.15084909263947066No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6950.1499858646415338No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6350.1370374446724805No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6280.13552679567609094No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6240.13466356767815407No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6230.13444776067866984No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6170.13315291868176451No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA6060.1307790416874381No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5970.1288367786920801No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5750.12408902470342723No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5660.12214676170806925No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5610.12106772671064814No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5400.1165357797214795No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAACGGGGCAGCA5340.11524093772457417No Hit
GTTCTATCCTAACCTTACTAAGTATTTGCCCTTAGACAAAGGCATTAAAC5270.11373028872818461No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5220.11265125373076351No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5050.10898253473953173No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5040.10876672774004753No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5030.1085509207405633No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG4820.10401897375139466No Hit
ATGTTAATATGGGCCTAAACATCAGACAACTATTGTGGTTTCACATTTCC4800.10358735975242621No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4750.1025083247550051No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4710.10164509675706822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)