FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463377
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40960.8839454698873703No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA15020.32414211322530034No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12200.2632845393707499No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT12190.26306873237126577No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10310.22249701646823214No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9870.2130015084909264No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9840.21235408749247375No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAC9810.2117066664940211No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9600.20717471950485242No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9570.20652729850639978No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8430.1819253005651985No Hit
GCCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7980.17221398558840859No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7530.16250267061161863No Hit
ATCCTAACCTTACTAAGTATTTGCCCTTAGACAAAGGCATTAAACCGTAT7260.15667588162554463No Hit
GTGTAAACGGGGCAGCAAAGCCTAAAAGACCCACAATTCTTTGACATACT7200.1553810396286393No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6760.14588553165133358No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6450.13919551466732272No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6280.13552679567609094No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6270.13531098867660674No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6210.1340161466797014No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6040.13034742768846963No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5950.12840516469311167No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5870.12667870869723788No Hit
GTTCTATCCTAACCTTACTAAGTATTTGCCCTTAGACAAAGGCATTAAAC5780.1247364457018799No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5750.12408902470342723No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5610.12106772671064814No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5470.11804642871786904No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5410.11675158672096371No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5280.11394609572766884No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG5260.11351448172870039No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5250.11329867472921616No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5150.11114060473437395No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5010.10811930674159485No Hit
ATGTTAATATGGGCCTAAACATCAGACAACTATTGTGGTTTCACATTTCC4920.10617704374623688No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT4880.10531381574829998No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4880.10531381574829998No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4850.1046663947498473No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4840.1044505877503631No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4770.10293993875397354No Hit
TCTCCTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT4660.10056606175964712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)