FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565007
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG27400.48494974398547275No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT24270.4295522002382271No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23400.4141541609219001No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC18600.3291994612456129No Hit
TATTTAGTGTGGGTAGGATAAAATCTAGCAGGCATAATTAATTTCAATCT17160.3037130513427267No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT16240.28743006723810505No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT14660.2594658119279938No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC13700.24247487199273637No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13680.2421208940774185No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA12950.22920070016831648No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA12860.2276077995493861No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC12850.2274308105917272No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG12730.22530694309982002No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT12670.22424500935386643No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT12240.21663448417453235No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG12000.212386749190718No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG11920.21097083752944654No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTCAGAAAACTTCCTGTTA11380.20141343381586424No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG10830.19167904114462297No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT10720.18973216261037473No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10600.18760829511846755No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10460.1851304497112425No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10450.18495346075358357No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA10370.18353754909231212No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAAAATCTAGCAGGCATAA10080.1784048693202031No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG9560.16920144352193867No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT9430.16690058707237257No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA9420.16672359811471363No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA8660.15327243733263482No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA8650.1530954483749759No Hit
ATTTTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8560.15150254775604552No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG8550.15132555879838655No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT8490.15026362505243296No Hit
GGATAAAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC8330.14743180172989007No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC8150.1442460004920293No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8090.14318406674607573No Hit
ATATTGTACAAAAGATCAAACACTGTTTCAGAAAACTTCCTGTTAACAGG7860.13911332071992027No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG7760.13734343114333097No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT7540.13344967407483446No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTGAAACAGTGTTTGATCTTTTGT7530.13327268511717555No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCGCCATGC7410.13114881762526837No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC7350.13008688387931477No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7070.1251311930648647No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT7070.1251311930648647No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7000.12389227036125217No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6910.12229936974232178No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6800.12035249120807352No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG6780.11999851329275568No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA6720.11893657954680209No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6710.11875959058914314No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT6700.11858260163148421No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTGAAAC6660.1178746458008485No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6470.1145118556053288No Hit
ATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTC6470.1145118556053288No Hit
GAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA6160.10902519791790191No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT6140.10867122000258404No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT5950.10530842980706434No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5890.10424649606111075No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT5810.10283058439983929No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5770.10212262856920357No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5720.10123768378090892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)