FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences565007
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG26100.4619411794898116No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23350.4132692161336054No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT23190.41043739281106256No Hit
TATTTAGTGTGGGTAGGATAAAATCTAGCAGGCATAATTAATTTCAATCT16620.2941556476291444No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT16310.2886689899417175No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC15570.2755718070749566No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT14540.2573419444360866No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTCAGAAAACTTCCTGTTA14180.25097034196036505No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC13960.2470765848918686No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT13630.24123594928912384No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG13320.235749291601697No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA13210.2338024130674487No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG12990.22990865599895222No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA12840.22725382163406826No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT12730.22530694309982002No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAAAATCTAGCAGGCATAA12560.22229813081961813No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG11590.20513020192670178No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG11460.2028293454771357No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC11370.2012364448582053No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11160.19751967674736773No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10750.19026312948335153No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA10010.17716594661659057No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10000.17698895765893166No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG9850.17433412329404768No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9660.170971333098528No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA9660.170971333098528No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG9480.16778553186066722No Hit
ATATTGTACAAAAGATCAAACACTGTTTCAGAAAACTTCCTGTTAACAGG9390.16619263124173683No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9370.16583865332641898No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT9140.16176790730026352No Hit
GGCCTATCTCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACGGGGCG9020.15964403980835634No Hit
ATTTTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC8920.15787415023176704No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA8790.15557329378220092No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC8610.15238749254434014No Hit
GGATAAAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC8050.14247611091544No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT7930.1403522434235328No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7870.13929030967757922No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7860.13911332071992027No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC7800.13805138697396668No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7600.13451160782078805No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT7490.1325647292865398No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT7350.13008688387931477No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7270.1286709722180433No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7070.1251311930648647No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG6940.12283033661529857No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG6910.12229936974232178No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG6730.119113568504461No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT6350.11238798811342161No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA6350.11238798811342161No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA6320.11185702124044482No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTTTAT6280.11114906540980907No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6270.11097207645215015No Hit
GTACAAAAGATCAAACACTGTTTCAGAAAACTTCCTGTTAACAGGCCTAT6240.11044110957917336No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTGAAACAGTGTTTGATCTTTTGT6230.11026412062151443No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT6120.10831724208726617No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC6040.10690133042599473No Hit
ATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTC5960.10548541876472327No Hit
GTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGTAGGATAAAAT5850.10353854023047503No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC5820.10300757335749823No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5810.10283058439983929No Hit
AGATAGGCCCAAGCGGCCCCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG5720.10123768378090892No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5670.10035273899261425No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT5670.10035273899261425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)