FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624981
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA47630.7621031679363053No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC43700.6992212563261924No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41680.6669002737683225No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20030.32048974288818377No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT18940.30304921269606594No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT17080.2732883079645621No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC16510.2641680307081335No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16190.25904787505540167No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15420.24672750051601566No Hit
TCCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT13440.2150465374147374No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT13070.20912635744126623No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG12620.20192613855461206No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT12490.19984607532068976No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC11160.17856542838902303No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10970.1755253359702135No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG10790.17264524841555182No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC10770.17232523868725608No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT10700.171205204638221No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10630.1700851705891859No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10540.1686451268118551No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC10460.1673650878986721No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTACGCATGTAT10380.16608504898548918No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT10330.16528502466474979No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10280.16448500034401045No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10230.16368497602327112No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG10180.16288495170253176No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10020.16032487387616584No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT9920.15872482523468714No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT9870.15792480091394778No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT9730.1556847328158776No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9640.15424468903854677No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAATCTAATCTCCTCC9560.1529646501253638No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA9120.14592443610285752No Hit
CATTTACACCAAGATATTATTAAAAAATGTGAACAATTTGTAGGCCCACT8910.14256433395575227No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC8890.14224432422745653No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8790.1406442755859778No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8440.13504410534080236No Hit
GTCAATGAGAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC8340.13344405669932366No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC8190.1310439837371056No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8080.12928393023147905No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7980.12768388159000033No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7930.126883857269261No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7880.12608383294852163No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7850.125603818356078No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT7820.1251238037636344No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7650.12240372107312063No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7650.12240372107312063No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG7620.12192370648067702No Hit
CCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC7590.12144369188823341No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT7500.12000364811090257No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7440.11904361892601535No Hit
GAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA7390.11824359460527599No Hit
TTGTAGGCCCACTCACAGTCAATGAGAAAAGAAGACTGCAATTGATTATG7390.11824359460527599No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG7310.11696355569209305No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT7300.11680355082794518No Hit
GAATACATGCGTACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7240.11584352164305795No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC7170.11472348759402286No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7000.11200340490350906No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6900.11040335626203035No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6710.10736326384322083No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6670.10672324438662935No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT6500.10400316169611556No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6480.10368315196781983No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6480.10368315196781983No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC6480.10368315196781983No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6480.10368315196781983No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT6430.10288312764708048No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAATCTAATCT6400.10240311305463685No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT6390.10224310819048898No Hit
ACCTAGTAGTTAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAA6280.10048305468486243No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6270.10032304982071456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)