FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462757
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34190.7388326918879671No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT17030.36801172105446267No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT13220.28567909291485594No Hit
CATTTAAACCCTAACAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT11960.2584509796718364No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG10260.22171463640744496No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9770.21112592570182626No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAACCAAACGTTGGG9450.20421084932264666No Hit
CCCTTTACACAATGTGGTTACCCAGCTTTAATGCCATTGTATGCATGTAT9210.19902454203826198No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG8640.1867070622378484No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8490.18346562018510795No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7970.17222862106894113No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7960.1720125249320918No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG7580.16380087173181604No Hit
GTATACATGCATACAATGGCATTAAAGCTGGGTAACCACATTGTGTAAAG7480.16163991036332243No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTGTTAGGGTTTAAATGTATAC7150.1545087378472935No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7130.1540765455735948No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6940.1499707189734569No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA6890.1488902382892101No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6760.1460809885101684No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6580.14219125804687988No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6410.13851762372044074No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6190.13376350870975479No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA5860.12663233619372585No Hit
CATGCATACAATGGCATTAAAGCTGGGTAACCACATTGTGTAAAGGGAGC5780.12490356709893098No Hit
CATTTAAAAGAGGATATTATCAATAGATGTCAACAATATGTGGGCCCTCT5690.12295870186728672No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC5650.12209431731988926No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT5640.12187822118303993No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCAC5530.11950116367769695No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTGTTAGGGTTTAAATGTATA5320.11496314480386033No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAA5310.11474704866701099No Hit
GCACTAAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4870.10523881864563907No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4830.10437443409824163No Hit
GCACAGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4830.10437443409824163No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC4670.10091689590865185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)