FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462757
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33930.7332141923298837No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT17420.3764394703915878No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT13280.2869756697359521No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG10410.2249560784601854No Hit
CATTTAAACCCTAACAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT10360.22387559777593855No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9800.2117742141123743No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAACCAAACGTTGGG9380.20269817636470114No Hit
CCCTTTACACAATGTGGTTACCCAGCTTTAATGCCATTGTATGCATGTAT9160.19794406135401516No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG9010.19470261930127475No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG8900.1923255617959318No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8450.1826012356377105No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7710.16661012151085774No Hit
GTATACATGCATACAATGGCATTAAAGCTGGGTAACCACATTGTGTAAAG7540.16293648718441858No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7440.16077552581592497No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7360.1590467567211301No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTGTTAGGGTTTAAATGTATAC6720.14521660396277095No Hit
ATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6600.14262345032057863No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6540.14132687349948245No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6440.13916591213098883No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA6090.13160254734126117No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT6060.1309542589307131No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6030.13030597052016502No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6020.13008987438331565No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC5600.12101383663564247No Hit
CATTTAAAAGAGGATATTATCAATAGATGTCAACAATATGTGGGCCCTCT5490.11863677913029949No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA5340.11539533707755907No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCAC5320.11496314480386033No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5060.10934464524577694No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACCAAACGTTGGGGATACT4970.10739978001413268No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4890.10567101091933778No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTGTTAGGGTTTAAATGTATA4890.10567101091933778No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4830.10437443409824163No Hit
GTACAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCC4770.10307785727714545No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAAC4760.10286176114029609No Hit
GCACAGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4730.10221347272974801No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4650.10048470363495311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)