FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420458
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15020.3572294973576433No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14330.3408188213804946No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACCACCGTG14160.3367756113571391No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT11760.27969499926270874No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT11570.2751761174718997No Hit
CATTTACACCAAGACATTATCAAAAAGTGTGAACAATTTGTAGGTCCACT11100.2639978309367404No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGACAGAGAGTCCAA10110.24045207844778788No Hit
ATATAATACACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGA9400.22356573070318558No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTTCACCAAACTCTGCAAG9330.221900879517098No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9300.22118737186591764No Hit
CCTCCAGCTTATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA9210.2190468489123765No Hit
CTCTTATATAATACACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGT8990.21381445947038705No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCAT8860.21072259298193877No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTACGCCTGTATT8820.20977124944703157No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA8630.2052523676562225No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8460.20120915763286704No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8410.20001997821423304No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC8320.19787945526069192No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8030.19098221463261492No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7920.1883660199116202No Hit
GTCATAGAGTATTTGGTGTCCTTCGGAGTGTGGATTCGCACTCCTCCAGC7910.18812818402789339No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7880.18741467637671302No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7820.18598766107435225No Hit
GGAAGATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGAAAAGT7570.18004176398118243No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTACTGACTTCTATCCGTCGGTACG7550.17956609221372882No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7490.17813907691136807No Hit
CTCTGTGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACGTAGCGCC7300.173620195120559No Hit
GTATTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC7150.17005265686465712No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7150.17005265686465712No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATACTGGATCTTC7110.16910131332974995No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG7050.16767429802738917No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7020.1669607903762088No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGT6990.16624728272502842No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6980.16600944684130162No Hit
CACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAGTGT6960.16553377507384806No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT6840.16267974446912653No Hit
ATCAAAAAGTGTGAACAATTTGTAGGTCCACTCACAGTAAATGAGAAACG6590.1567338473759567No Hit
ATGCAAACCCTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC6530.15530683207359594No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC6410.1524528014688744No Hit
AAGAAGGTGTTTTCCAATGAGGGTTAAAGACAGGTACAGTAGAAGAATAA6390.15197712970142085No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6190.14722041202688496No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6180.14698257614315818No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6140.14603123260825102No Hit
GGGTAAAGGGTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5970.1419880225848955No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5900.14032317139880796No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5880.13984749963135437No Hit
CACCAAGACATTATCAAAAAGTGTGAACAATTTGTAGGTCCACTCACAGT5830.1386583202127204No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG5770.13723130491035967No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5700.1355664537242721No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5630.13390160253818453No Hit
CTTATATAATACACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTT5620.13366376665445776No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5540.13176107958464342No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG5530.13152324370091661No Hit
GTGTAAACAATACCTGACCCTTTACCCCGTTGCTAGGCAACGGCCAGGTC5530.13152324370091661No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5460.12985839251482909No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5310.12629085425892717No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA5280.12557734660774678No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5250.1248638389565664No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5220.12415033130538602No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5200.12367465953793245No Hit
GATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT5180.12319898777047886No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT5090.12105846481693772No Hit
CCCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCT5080.12082062893321094No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC5070.12058279304948413No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5050.12010712128203054No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAACCCCAGCCCGAATGCTCCAG5040.11986928539830376No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5030.11963144951457697No Hit
GGCTATGTCATTGGATGTTATGGGTCCTTACCACAAGATCACATCAGACA5030.11963144951457697No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4970.11820443421221621No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4940.11749092656103581No Hit
GGCCTATTGATTGGAAAGTTTGTCAACGTATTGTGGGTCTTTTGGGTTTT4890.11630174714240187No Hit
CCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4890.11630174714240187No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC4870.11582607537494827No Hit
CTCTGGGATCTTGCAGAGTTTGGTGAAAAGTTGTGGAGTTCCACTGCATG4830.1148747318400411No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGGTCAGGTATTGTTTACAC4780.11368555242140713No Hit
CTTTTAGATACCGCCTCAGCTCTGTATCGAGATGCCTTGGAGTCTCCTGA4730.11249637300277317No Hit
GTATCATCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4680.1113071935841392No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4670.1110693577004124No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAAT4670.1110693577004124No Hit
GAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTTTCCGAAAG4670.1110693577004124No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG4660.11083152181668562No Hit
GCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4640.11035585004923203No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG4630.11011801416550522No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGGCAGAATCTTTCCACCA4600.10940450651432485No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4590.10916667063059805No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4580.10892883474687128No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTACGCCTGTATTCAATCTAAGC4550.10821532709569089No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG4550.10821532709569089No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAGGACACCAAATACTCTATGA4500.10702614767705693No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4490.10678831179333013No Hit
CTCCGAAGGACACCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGA4490.10678831179333013No Hit
GTATCGAGATGCCTTGGAGTCTCCTGAACATTGTACACCTCACCATACTG4470.10631264002587655No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4450.10583696825842295No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA4380.10417211707233541No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG4330.10298293765370144No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT4330.10298293765370144No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGTCTCTA4320.10274510176997463No Hit
GACATAGCCCATGAAATTTAAAGAATAACCCCATCTTTTAGTTTTGTTAG4280.10179375823506746No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4270.10155592235134069No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTCATGTCAACGACCGACCT4260.1013180864676139No Hit
GCTTTAATGCCCTTGTACGCCTGTATTCAATCTAAGCAGGCTTTCACTTT4240.10084241470016031No Hit
ACCATATTCTTGGGAACAAGATCTACAGCATGGGGCAGAATCTTTCCACC4220.10036674293270671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)