FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420458
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACCACCGTG16470.39171570049802834No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13760.3272621760080674No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13460.3201270994962636No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT12140.2887327628443269No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT11040.2625708156343797No Hit
ATATAATACACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTTGGA10270.24425745258741657No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTTCACCAAACTCTGCAAG9740.23165215074989653No Hit
CATTTACACCAAGACATTATCAAAAAGTGTGAACAATTTGTAGGTCCACT9540.2268954330753607No Hit
CTCTTATATAATACACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGT9460.22499274600554633No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA9410.22380356658691236No Hit
CCTCCAGCTTATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA9370.2228522230520052No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGACAGAGAGTCCAA9090.216192818307655No Hit
GTCATAGAGTATTTGGTGTCCTTCGGAGTGTGGATTCGCACTCCTCCAGC9010.21429013123784066No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8840.21024692121448516No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCAT8750.20810639826094401No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8640.20549020353994926No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG8600.20453886000504212No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT8410.20001997821423304No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTACGCCTGTATT8340.19835512702814548No Hit
GTATCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA8240.1959767681908776No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8100.19264706581870247No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC7940.18884169167907378No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7910.18812818402789339No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7710.18337146635335752No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7450.1771877333764609No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7260.17266885158565184No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATACTGGATCTTC7200.17124183628329107No Hit
GTATTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACC7180.17076616451583748No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7000.16648511860875523No Hit
CTCTGTGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACGTAGCGCC6970.16577161095757484No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC6880.1636310880040337No Hit
GGAAGATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGAAAAGT6840.16267974446912653No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6790.16149056505049256No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTACTGACTTCTATCCGTCGGTACG6370.15150145793396724No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG6350.15102578616651366No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6240.14840959144551893No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC6130.1457933967245242No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACCTAATGT5950.14151235081744193No Hit
CACCCTCTTTTCCTAATATACATTTACACCAAGACATTATCAAAAAGTGT5940.14127451493371512No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5920.14079884316626157No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT5840.1388961560964472No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5830.1386583202127204No Hit
ATGCAAACCCTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC5770.13723130491035967No Hit
CTTATATAATACACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTT5760.13699346902663287No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5740.13651779725917926No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5720.1360421254917257No Hit
GGGTAAAGGGTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5640.13413943842191134No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAACCCCAGCCCGAATGCTCCAG5570.13247458723582378No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT5530.13152324370091661No Hit
AAGAAGGTGTTTTCCAATGAGGGTTAAAGACAGGTACAGTAGAAGAATAA5460.12985839251482909No Hit
GTGTAAACAATACCTGACCCTTTACCCCGTTGCTAGGCAACGGCCAGGTC5430.1291448848636487No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5410.1286692130961951No Hit
CCCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCT5380.12795570544501472No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG5370.12771786956128792No Hit
ATCAAAAAGTGTGAACAATTTGTAGGTCCACTCACAGTAAATGAGAAACG5340.12700436191010755No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5280.12557734660774678No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5250.1248638389565664No Hit
CTTTTAGATACCGCCTCAGCTCTGTATCGAGATGCCTTGGAGTCTCCTGA5190.12343682365420566No Hit
GGCCTATTGATTGGAAAGTTTGTCAACGTATTGTGGGTCTTTTGGGTTTT5100.12129630070066451No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT5100.12129630070066451No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5040.11986928539830376No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5040.11986928539830376No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG4980.11844227009594299No Hit
GCGCGGGACCATGCAGAACCTGCACGACTACTGCTCAAGGAACCTCTATG4950.11772876244476262No Hit
GTATCATCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA4950.11772876244476262No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4920.11701525479358224No Hit
GGCTATGTCATTGGATGTTATGGGTCCTTACCACAAGATCACATCAGACA4900.11653958302612864No Hit
CCTGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4890.11630174714240187No Hit
GATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT4860.11558823949122148No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGGTCAGGTATTGTTTACAC4840.11511256772376789No Hit
GCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4830.1148747318400411No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG4800.11416122418886072No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4770.11344771653768035No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4710.11202070123531957No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4700.11178286535159279No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4700.11178286535159279No Hit
CTCTGGGATCTTGCAGAGTTTGGTGAAAAGTTGTGGAGTTCCACTGCATG4680.1113071935841392No Hit
GTATCGAGATGCCTTGGAGTCTCCTGAACATTGTACACCTCACCATACTG4610.10964234239805165No Hit
CACCAAGACATTATCAAAAAGTGTGAACAATTTGTAGGTCCACTCACAGT4610.10964234239805165No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG4600.10940450651432485No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA4590.10916667063059805No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC4530.1077396553282373No Hit
CTCCGAAGGACACCAAATACTCTATGACTGTTTCTCTTCCAAAAGTGAGA4530.1077396553282373No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTACGCCTGTATTCAATCTAAGC4520.10750181944451051No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4480.10655047590960333No Hit
GTATTTGGTGTCCTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGAC4460.10607480414214976No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAGGACACCAAATACTCTATGA4440.10559913237469616No Hit
GAATACAGGCGTACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4400.10464778883978898No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4380.10417211707233541No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT4340.10322077353742823No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC4280.10179375823506746No Hit
CTCCTGAACATTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTT4270.10155592235134069No Hit
ATCCCAGAGTGAGAGGCCTGTATCTTCCTGCTGGTGGCTCCAGTTCAGGA4270.10155592235134069No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4260.1013180864676139No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4260.1013180864676139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)