FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377998
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14990.3965629447774856No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14800.39153646315588975No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG14550.3849226715485267No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC14040.37143053666950615No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGAAGTCCAA13120.34709178355441034No Hit
ATTAATAGGTGTCAACAGTTTGTGGGCCCTCTCACTGTAAATGAAAAGAG11710.30978999888888303No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC11690.30926089556029396No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG11160.2952396573526844No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10750.2843930391166091No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT10710.28333483245943103No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA10710.28333483245943103No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT10520.2783083508378351No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA9660.25555690770850636No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT8780.23227636125058862No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8650.22883718961475988No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT8260.21851967470727357No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7780.20582119482113662No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC7670.2029111265138969No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA7550.19973650654236266No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7440.1968264382351229No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC7430.19656188657082843No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT7290.19285816327070515No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7140.18888988830628733No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC7140.18888988830628733No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC7060.18677347499193117No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6860.18148244170604078No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6650.17592685675585584No Hit
GTGGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAATACCGCCGTG6330.16746120349843122No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6280.1661384451769586No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6100.16137651521965724No Hit
ATTAACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT6000.15873099857671205No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG5920.1566145852623559No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5920.1566145852623559No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT5920.1566145852623559No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5840.15449817194799972No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5740.15185265530505454No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG5740.15185265530505454No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT5690.15052989698358193No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA5580.1476198286763422No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5530.1462970703548696No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5470.14470976036910246No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5440.1439161053762189No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG5440.1439161053762189No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC5420.14338700204762989No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5400.14285789871904084No Hit
GTATCGAGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTG5370.14206424372615728No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA5330.1410060370689792No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5300.14021238207609565No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA5280.1396832787475066No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5270.1394187270832121No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT5260.13915417541891756No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA5240.1386250720903285No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5200.13756686543315044No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC5160.13650865877597237No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT5130.13571500378308882No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5130.13571500378308882No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5080.1343922454616162No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5080.1343922454616162No Hit
TTATTAATAGGTGTCAACAGTTTGTGGGCCCTCTCACTGTAAATGAAAAG5070.13412769379732167No Hit
GTACTGTAGAGGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA5070.13412769379732167No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG5030.1330694871401436No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA5020.13280493547584907No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4930.1304239704971984No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT4880.1291012121757258No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG4820.12751390218995867No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4790.12672024719707511No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4790.12672024719707511No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG4770.12619114386848607No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT4740.1253974888756025No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT4720.12486838554701349No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4690.12407473055412993No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA4650.12301652389695183No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC4590.1214292139111847No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC4570.12090011058259569No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT4460.11799004227535596No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA4430.1171963872824724No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTC4410.11666728395388336No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCCGTAAAGTTTCCCAC4390.11613818062529431No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4380.11587362896099979No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4370.11560907729670528No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4360.11534452563241075No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC4360.11534452563241075No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4330.11455087063952718No Hit
GTACAAAACCTATGGATGGAAATTGCACCTGTATTCCCATCCCATCGTCC4320.11428631897523267No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG4300.11375721564664362No Hit
ATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGACTCTA4280.1132281123180546No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4270.11296356065376006No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAG4260.11269900898946555No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4250.11243445732517104No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC4220.11164080233228749No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT4220.11164080233228749No Hit
GTAGAGGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT4190.11084714733940393No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG4170.11031804401081488No Hit
GTCTTACATAAGAGGACTCTTGGACTTCCAGCAATGTCAACGACCGACCT4170.11031804401081488No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4150.10978894068222583No Hit
GTCCAACTGATGATCGGGAAAGAATCCCAGAGGGTTGGGAACAGAAAGAT4140.10952438901793132No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4120.10899528568934228No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA4100.10846618236075323No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4050.10714342403928064No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT4040.1068788723749861No Hit
CTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACT4000.10582066571780803No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC3970.10502701072492447No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT3960.10476245906062996No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3920.10370425240345187No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3910.10343970073915736No Hit
TCACCATACTGCACTCAGGCAAGCCATTCTCTGCTGGGGGGAATTGATGA3890.10291059741056831No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT3880.1026460457462738No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT3800.10052963243191762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)