FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219705
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT9780.44514234996927704No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8950.40736442047290683No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCTGAAAGTCCAA8790.40008192803987164No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8290.37732413918663665No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATA8120.36958649097653673No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA7400.3368152750278783No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC6620.30131312441683167No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG6330.28811360688195536No Hit
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT6120.27855533556359663No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT6080.2767347124553378No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT6060.27582440090120847No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC5920.2694522200223026No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG5650.2571630140415557No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA5610.2553423909332969No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC5610.2553423909332969No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA5490.2498805216085205No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT4840.22029539609931498No Hit
GTTGTAAGCACACCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTG4600.20937165744976222No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT4260.19389636102956237No Hit
CCTTTATACCGCTGTTACCAATTTTCTGTTATCTGTGGGTATCCATTTAA4230.19253089369836826No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4200.19116542636717415No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC4020.18297262238000955No Hit
TAAATACACAGTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCT4010.18251746660294485No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC3970.18069684349468607No Hit
CCCATAGGTATTTTCCTAAAGCCCAGGATGATGGGATGGGAATACAAGTG3960.18024168771762136No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCAGGAGGAGTGCGAATCCACACT3910.17796590883229785No Hit
GGGTAAAGGTTCATGTATTGTTGACATAAAAAGGCCTTGTAAGTTGGCGA3880.17660044150110377No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGAGACACAGAACCCTGCTCCGA3850.17523497416990966No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC3740.17022826062219795No Hit
ATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAATAA3630.16522154707448625No Hit
GTCCCGTGCTGGTGGTCGTGGATCCTGGAAGTAGAGGACAAACGGGCAAC3600.16385607974329214No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA3590.16340092396622743No Hit
ATACCAGATCATCCATATAACTAAAAGCCAGACAGTGGGGGAAAGCCCTA3580.16294576818916273No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTG3560.16203545663503335No Hit
TCCACAGGCCATGCAGTGGAACTCCACTCAGTTCCACCAGGCTCTGTTAG3550.16158030085796862No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG3550.16158030085796862No Hit
CTATTTGCATACTTTATGGAAAGCAGGCATTCTATATAAGAGAGAATCCA3440.15657358731025695No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC3430.15611843153319224No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3430.15611843153319224No Hit
GTAGAGGAATAGAGTCCCGTAAAATTTCCCACCTTATGAGTCCAAGGAAT3400.15475296420199813No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG3400.15475296420199813No Hit
CCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTGAGGGGGTGTGAA3380.15384265264786873No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC3370.15338749687080405No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT3360.15293234109373932No Hit
GCTTACAACATTGCCAGCAAATCCGCCTCCTGCTTCCACCAATCGTCGGT3360.15293234109373932No Hit
CAATATAGCTTGCCTGAGAGCGGTATGGTTAGGTGTGCAATGTTCTGGTG3320.15111171798548054No Hit
GTTATACAGGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAA3280.14929109487722173No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT3260.14838078332309232No Hit
GGCACAGGGTGTGCTTACAACATTGCCAGCAAATCCGCCTCCTGCTTCCA3260.14838078332309232No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCCACGACCACC3220.14656016021483353No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC3180.14473953710657472No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3180.14473953710657472No Hit
CCTCTATTCAGGGCAAATTCCAGCAGTCCCGACTGGGACTTCAACAAAAA3130.14246375822125124No Hit
GAATACTAACATTGGGAAGCTGGAGATTGAGATCTTCTGCGACGCGGCGA3090.14064313511299242No Hit
TGTTAGTATTGACATAGTTAACCACTAGGTCCCTAGCAGCAGGATCTTCC2980.13563642156528072No Hit
GGAAAATACAGAGCCCTTACCCTCGGATCTAACAGAGCCTGGTGGAACTG2950.1342709542340866No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTATAGACCACCAAATGCC2920.1329054869028925No Hit
GTTAACCACTAGGTCCCTAGCAGCAGGATCTTCCAAGTTATTGCCCACCC2860.1301745522405043No Hit
ACATAGAGGTTCCTTGAGCAAGAGTTGTGCAGGTTTTGCATGGTCCCGTG2840.1292642406863749No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCC2840.1292642406863749No Hit
GTCTTACATAAGCGGACTCTTGGACTTTCAGGAAGGTCAATCACCTGGAT2820.1283539291322455No Hit
GCTAGGGACCTAGTGGTTAACTATGTCAATACTAACATGGGCCTAAAAAT2810.12789877335518082No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCT2800.1274436175781161No Hit
GTCCGGCAGATGAGAAGGAACAGACGGGGAGGCCGCGTAAAGAGAGGTGC2790.12698846180105142No Hit
GTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCTTCTGCGAC2770.126078150246922No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2740.12471268291572792No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT2730.1242575271386632No Hit
ATTTAAATGGATACCCACAGATAACAGAAAATTGGTAACAGCGGTATAAA2720.12380237136159851No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT2660.1210714366992103No Hit
GTGGATGAGTGTCTCTTAGAGGTGGAGAGACTGGGGTTGGTTTTCTCCCG2620.11925081359095151No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG2620.11925081359095151No Hit
GTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAATAACTTGG2610.11879565781388679No Hit
GCCATGGGAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGTTGTCTGTG2600.1183405020368221No Hit
ATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA2590.11788534625975741No Hit
GGATTAAAAGTAGGAACAGTAGAGGAATAGAGTCCCGTAAAATTTCCCAC2580.1174301904826927No Hit
GTTATTGGCAGTTGGGGATCATTACCACAAGATCACATTGTACAAAAAAT2580.1174301904826927No Hit
GTGGGGGAAAGCCCTACGCACCACTGAACAAATTGCACTAGTAAACTGAG2580.1174301904826927No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2580.1174301904826927No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTG2570.11697503470562799No Hit
CTGTTAGATCCGAGGGTAAGGGCTCTGTATTTTCCTGCTGGTGGCTCCAG2560.1165198789285633No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTATTGTTGACAT2560.1165198789285633No Hit
ATTTGCATACTTTATGGAAAGCAGGCATTCTATATAAGAGAGAATCCACA2550.11606472315149859No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACCGCCTGTAACACG2540.1156095673744339No Hit
CTCCTACCTTGTTTGCCATTGGCCAATTGTCCTTGTTTTTGTTGAAGTCC2530.11515441159736921No Hit
GCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGAAACTAC2530.11515441159736921No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG2530.11515441159736921No Hit
GCCAATAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTT2500.1137889442661751No Hit
ACATGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTG2500.1137889442661751No Hit
GGTTCATGTATTGTTGACATAAAAAGGCCTTGTAAGTTGGCGAAAAGACA2450.11151316538085158No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2440.1110580096037869No Hit
CCTCTAAGAGACACTCATCCACAGGCCATGCAGTGGAACTCCACTCAGTT2440.1110580096037869No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2430.1106028538267222No Hit
GTTTCCGTCCGAGGGTTTGGAACAGCAGCAAGAGGGAAACATAGAGGTTC2430.1106028538267222No Hit
CCTCTATGTTTCCCTCTTGCTGCTGTTCCAAACCCTCGGACGGAAACTGC2430.1106028538267222No Hit
CCTAACCATACCGCTCTCAGGCAAGCTATATTGTGCTGGGGTGAGTTAAT2430.1106028538267222No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATC2430.1106028538267222No Hit
TAACCATACCGCTCTCAGGCAAGCTATATTGTGCTGGGGTGAGTTAATGA2420.11014769804965749No Hit
ATTCTGTCCCATGCCCCTTCGAGTCGTTGATAGAGGTGCTCCCATGCTGT2420.11014769804965749No Hit
ATCTGTGGGTATCCATTTAAATACCTCGAAAACAAAAAGATGGGGTTATA2400.1092373864955281No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT2400.1092373864955281No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGCACCTCTATCAACGA2400.1092373864955281No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG2370.107871919164334No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2360.10741676338726928No Hit
GGACAGAATCTCTCTGTACCCAATCCTCTGGGATTCTTTCCAGACCATCA2360.10741676338726928No Hit
CCTCTATCAACGACTCGAAGGGGCATGGGACAGAATCTCTCTGTACCCAA2320.10559614027901049No Hit
CTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA2290.10423067294781639No Hit
GATCTAACAGAGCCTGGTGGAACTGAGTGGAGTTCCACTGCATGGCCTGT2290.10423067294781639No Hit
GTCCTTAAATACACAGTCTTTGATGTATTCTTCGATCCAGGTGATTGACC2280.10377551717075169No Hit
CTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTT2260.10286520561662228No Hit
GAATACAAGTGCAGTTTCCGTCCGAGGGTTTGGAACAGCAGCAAGAGGGA2260.10286520561662228No Hit
GTTCGTAGCAGGGCCCCCAGTCTTCAAGAAGATTGATGATGTGAGAGAGG2240.10195489406249289No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTAGGAAAATACCTATGGGAG2230.10149973828542819No Hit
ACTTTATGGAAAGCAGGCATTCTATATAAGAGAGAATCCACACGTAGCGC2220.10104458250836348No Hit
GCATGGGAGCACCTCTATCAACGACTCGAAGGGGCATGGGACAGAATCTC2210.1005894267312988No Hit
GAATCTCTCTGTACCCAATCCTCTGGGATTCTTTCCAGACCATCAGCTGG2210.1005894267312988No Hit
CTCATCCACAGGCCATGCAGTGGAACTCCACTCAGTTCCACCAGGCTCTG2210.1005894267312988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)