FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219705
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT9770.44468719419221225No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8290.37732413918663665No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8190.3727725814159896No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATA7600.3459183905691723No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA7340.33408434036549006No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCTGAAAGTCCAA6990.31815388816822554No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT6930.3154229535058374No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG6830.3108713957351904No Hit
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT5860.26672128535991446No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC5690.2589836371498145No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC5570.25352176782503816No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT5510.25079083316264994No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA5480.24942536583145583No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA5100.23212944630299717No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG5050.22985366741767368No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT4510.2052752554561799No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGAGACACAGAACCCTGCTCCGA4190.19071027059010948No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC4150.18888964748185066No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC4130.18797933592772126No Hit
GTTGTAAGCACACCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTG4100.18661386859652715No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC4080.18570355704239774No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT3940.17933137616349196No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC3890.17705559727816844No Hit
CCTTTATACCGCTGTTACCAATTTTCTGTTATCTGTGGGTATCCATTTAA3830.17432466261578025No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG3760.17113857217632733No Hit
CCCATAGGTATTTTCCTAAAGCCCAGGATGATGGGATGGGAATACAAGTG3670.16704217018274503No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCAGGAGGAGTGCGAATCCACACT3650.16613185862861565No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC3630.16522154707448625No Hit
GTAGAGGAATAGAGTCCCGTAAAATTTCCCACCTTATGAGTCCAAGGAAT3590.16340092396622743No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC3550.16158030085796862No Hit
GTCCCGTGCTGGTGGTCGTGGATCCTGGAAGTAGAGGACAAACGGGCAAC3530.16066998930383924No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA3490.15884936619558046No Hit
CCTCTATTCAGGGCAAATTCCAGCAGTCCCGACTGGGACTTCAACAAAAA3490.15884936619558046No Hit
GTTTCCGTCCGAGGGTTTGGAACAGCAGCAAGAGGGAAACATAGAGGTTC3460.15748389886438635No Hit
ATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAATAA3440.15657358731025695No Hit
CCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTGAGGGGGTGTGAA3410.15520811997906284No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG3370.15338749687080405No Hit
TCCACAGGCCATGCAGTGGAACTCCACTCAGTTCCACCAGGCTCTGTTAG3320.15111171798548054No Hit
GGCACAGGGTGTGCTTACAACATTGCCAGCAAATCCGCCTCCTGCTTCCA3320.15111171798548054No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA3290.14974625065428643No Hit
GGGTAAAGGTTCATGTATTGTTGACATAAAAAGGCCTTGTAAGTTGGCGA3240.14747047176896294No Hit
CAATATAGCTTGCCTGAGAGCGGTATGGTTAGGTGTGCAATGTTCTGGTG3190.14519469288363943No Hit
GCTTACAACATTGCCAGCAAATCCGCCTCCTGCTTCCACCAATCGTCGGT3150.14337406977538064No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC3130.14246375822125124No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTG3110.1415534466671218No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCCACGACCACC3110.1415534466671218No Hit
CTCTTTACGCGGCCTCCCCGTCTGTTCCTTCTCATCTGCCGGACCGTGTG3100.14109829089005713No Hit
GTTATACAGGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAA3100.14109829089005713No Hit
GTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCTTCTGCGAC3040.1383673562276689No Hit
GAATACTAACATTGGGAAGCTGGAGATTGAGATCTTCTGCGACGCGGCGA3030.13791220045060423No Hit
CTATTTGCATACTTTATGGAAAGCAGGCATTCTATATAAGAGAGAATCCA3010.13700188889647483No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCT3000.13654673311941012No Hit
GGAAAATACAGAGCCCTTACCCTCGGATCTAACAGAGCCTGGTGGAACTG2960.1347261100111513No Hit
TAAATACACAGTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCT2940.1338157984570219No Hit
ATACCAGATCATCCATATAACTAAAAGCCAGACAGTGGGGGAAAGCCCTA2930.13336064267995723No Hit
TGTTAGTATTGACATAGTTAACCACTAGGTCCCTAGCAGCAGGATCTTCC2900.13199517534876312No Hit
GTCTTACATAAGCGGACTCTTGGACTTTCAGGAAGGTCAATCACCTGGAT2890.13154001957169842No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2880.13108486379463372No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2840.1292642406863749No Hit
CTGTTAGATCCGAGGGTAAGGGCTCTGTATTTTCCTGCTGGTGGCTCCAG2810.12789877335518082No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT2800.1274436175781161No Hit
GCTAGGGACCTAGTGGTTAACTATGTCAATACTAACATGGGCCTAAAAAT2780.1265333060239867No Hit
ATTTAAATGGATACCCACAGATAACAGAAAATTGGTAACAGCGGTATAAA2780.1265333060239867No Hit
CTCCTACCTTGTTTGCCATTGGCCAATTGTCCTTGTTTTTGTTGAAGTCC2730.1242575271386632No Hit
GTGGATGAGTGTCTCTTAGAGGTGGAGAGACTGGGGTTGGTTTTCTCCCG2730.1242575271386632No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG2720.12380237136159851No Hit
GGGCTGTATCGCCCCCTTCTCCGTCTGCCGTTCCAGCCGACGACGGGTCG2680.12198174825333971No Hit
GTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAATAACTTGG2680.12198174825333971No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2680.12198174825333971No Hit
ACATGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTG2660.1210714366992103No Hit
GTATCGCCCCCTTCTCCGTCTGCCGTTCCAGCCGACGACGGGTCGCACCT2660.1210714366992103No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTATAGACCACCAAATGCC2640.1201611251450809No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG2610.11879565781388679No Hit
GTTAACCACTAGGTCCCTAGCAGCAGGATCTTCCAAGTTATTGCCCACCC2600.1183405020368221No Hit
GTTATTGGCAGTTGGGGATCATTACCACAAGATCACATTGTACAAAAAAT2560.1165198789285633No Hit
GGATTAAAAGTAGGAACAGTAGAGGAATAGAGTCCCGTAAAATTTCCCAC2540.1156095673744339No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACCGCCTGTAACACG2530.11515441159736921No Hit
ATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA2520.1146992558203045No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTATTGTTGACAT2500.1137889442661751No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT2500.1137889442661751No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT2490.11333378848911041No Hit
GTCCTGGGTTCACACCCCCTCACGGTGGCCTGTTGGGGTGGAGTCCACAG2450.11151316538085158No Hit
CCTAACCATACCGCTCTCAGGCAAGCTATATTGTGCTGGGGTGAGTTAAT2450.11151316538085158No Hit
GTATTTAAATGGATACCCACAGATAACAGAAAATTGGTAACAGCGGTATA2450.11151316538085158No Hit
GCCAATAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTT2430.1106028538267222No Hit
ATTTGCATACTTTATGGAAAGCAGGCATTCTATATAAGAGAGAATCCACA2420.11014769804965749No Hit
CCTCTAAGAGACACTCATCCACAGGCCATGCAGTGGAACTCCACTCAGTT2420.11014769804965749No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTG2410.1096925422725928No Hit
GGTTCATGTATTGTTGACATAAAAAGGCCTTGTAAGTTGGCGAAAAGACA2410.1096925422725928No Hit
ATCTGTGGGTATCCATTTAAATACCTCGAAAACAAAAAGATGGGGTTATA2400.1092373864955281No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2390.10878223071846338No Hit
GAACAAATTGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC2380.10832707494139869No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG2380.10832707494139869No Hit
GTGGGGGAAAGCCCTACGCACCACTGAACAAATTGCACTAGTAAACTGAG2370.107871919164334No Hit
CCTCTATGTTTCCCTCTTGCTGCTGTTCCAAACCCTCGGACGGAAACTGC2360.10741676338726928No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2360.10741676338726928No Hit
GCCTAATCATCTTTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2350.1069616076102046No Hit
ACATAGAGGTTCCTTGAGCAAGAGTTGTGCAGGTTTTGCATGGTCCCGTG2340.10650645183313989No Hit
CCTTTACTCAATGTGGTTATCCTGCTCTCATGCCTCTGTATGCCTGTATA2330.10605129605607518No Hit
GTGGTTAACTATGTCAATACTAACATGGGCCTAAAAATTAGACAATTGCT2310.1051409845019458No Hit
GATCTAACAGAGCCTGGTGGAACTGAGTGGAGTTCCACTGCATGGCCTGT2310.1051409845019458No Hit
GCATGGGAGCACCTCTATCAACGACTCGAAGGGGCATGGGACAGAATCTC2310.1051409845019458No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC2310.1051409845019458No Hit
CTCATCCACAGGCCATGCAGTGGAACTCCACTCAGTTCCACCAGGCTCTG2300.10468582872488111No Hit
GAATCTCTCTGTACCCAATCCTCTGGGATTCTTTCCAGACCATCAGCTGG2280.10377551717075169No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2270.103320361393687No Hit
ATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGAT2270.103320361393687No Hit
GGGTTATACCCTAAATTTCATGGGTTATGTTATTGGCAGTTGGGGATCAT2260.10286520561662228No Hit
GGTTGGGGCTTGGCCATTGGCCATCAGCGCATGCGTGGAACCTTTGTGGC2260.10286520561662228No Hit
GTACAAAAAATCAAAGATTGTTTTCGTAAACTTCCTGTAAATCGCCCTAT2250.1024100498395576No Hit
GCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGAAACTAC2240.10195489406249289No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT2240.10195489406249289No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTAGGAAAATACCTATGGGAG2240.10195489406249289No Hit
TAACCATACCGCTCTCAGGCAAGCTATATTGTGCTGGGGTGAGTTAATGA2230.10149973828542819No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATC2230.10149973828542819No Hit
ACCCAGGACCGTATCCTCCCACTCCTACCTTGTTTGCCATTGGCCAATTG2220.10104458250836348No Hit
GTATAACCCCATCTTTTTGTTTTCGAGGTATTTAAATGGATACCCACAGA2220.10104458250836348No Hit
AGGTAGGAGTGGGAGGATACGGTCCTGGGTTCACACCCCCTCACGGTGGC2200.10013427095423408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)