FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439865
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT26000.5910904482056994No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT24920.566537460357155No Hit
GGAAAATATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTT23230.5281165812237846No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT16830.38261739397315087No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15530.35306287156286587No Hit
GGAAAGTATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTT14340.3260091164334512No Hit
CCTTTATACCGCTGTTACCAATTTTCTGTTATCTGTGGGTATCCATTTAA14000.31827947211076124No Hit
AATACTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAACGTG11950.2716742636945426No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCCGAAAGTCCAA11830.2689461539335932No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC11800.2682641264933559No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG11590.2634899344116945No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT11420.25962511225034957No Hit
CTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTT11250.2557602900890046No Hit
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT10620.24143771384402035No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG10620.24143771384402035No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC10530.2393916315233083No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT10300.23416275448148866No Hit
ATACTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAACGTGG10270.2334807270412513No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA10000.22734248007911517No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC10000.22734248007911517No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT9960.22643311015879872No Hit
ATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGAT9700.22052220567674174No Hit
ATATTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAACGTGG9680.22006752071658348No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC9210.2093824241528651No Hit
GTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGG9180.20870039671262774No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG8540.19415047798756435No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATA8520.19369579302740614No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT8500.1932411080672479No Hit
CCATAAAGTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGT8430.1916497107066941No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA8120.18460209382424153No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC7700.17505370966091868No Hit
ACTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTT7280.16550532549759586No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG7270.16527798301751673No Hit
GAAAATATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTTT7150.16254987325656736No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTG7030.15982176349561797No Hit
CCTCTATTCAGGGCAAATTCCAGCAGTCCCGACTGGGACTTCAACAAAAA6810.15482022893387742No Hit
AATGTGAACAATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTA6720.1527741466131654No Hit
ATTTTAATCTTCTCAATTCATTTTTTGTGAGAGGGCCTACAAATTGTTCA6630.15072806429245336No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6600.150046036852216No Hit
GGGTAAAGGTTCATGTATTGTTGACATAGAAAGGCCTTGTAAGTTGGCGA6590.1498186943721369No Hit
CTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTGTTCCTACT6590.1498186943721369No Hit
GTCCCGTGCTGGTGGTCGTGGATCCTGGAAGTAGAGGACAAACGGGCAAC6390.1452718447705546No Hit
GCCAATAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTT6370.1448171598103964No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA6320.1436804474100008No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC6280.14277107748968432No Hit
GATTAAAATTAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATAT6280.14277107748968432No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA6270.14254373500960524No Hit
ACACAAATGTGAACAATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAA6250.142089050049447No Hit
CCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCT6140.1395882827685767No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG6140.1395882827685767No Hit
CATTTACATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCCCTCT6110.1389062553283394No Hit
GTCTTACATAAGCGGACTCTTGGACTTTCGGGAAGGTCAATCACCTGGAT6070.1379968854080229No Hit
GTCTTAAAATAATGATTAACCACGTTATCAGGATAATAGGGTTTAATTCC5970.13572346060723176No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC5960.13549611812715265No Hit
ATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA5950.13526877564707354No Hit
TTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTAAAATTAGTTATG5940.1350414331669944No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTC5830.13254066588612415No Hit
GCTAGGGACCTAGTGGTTAACTATGTCAATACTAACATGGGCCTAAAAAT5820.132313323406045No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAGTATTTGGTAACCTT5820.132313323406045No Hit
CAATATAGCTTGCCTGAGAGCGGTATGGTTAGGTGTGCAATGTTCTGGTG5790.1316312959658077No Hit
GCCATGGGAAGGAGGTGTACTTCCTAGAGAGGACAACAGAGTTGTCTGTG5720.1300398986052539No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA5690.12935787116501654No Hit
CTGTACGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA5690.12935787116501654No Hit
GTATGGATCGGTAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5610.1275391313243836No Hit
ATCTGTGGGTATCCATTTAAATACCTCGAAAACAAAAAGATGGGGTTATA5600.1273117888443045No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC5540.1259477339638298No Hit
ATACCAGATCATCCATATAACTAAAAGCCAGACAGTGGGGGAAAGCCCTA5490.12481102156343424No Hit
GTGGTTAACTATGTCAATACTAACATGGGCCTAAAAATTAGACAATTGCT5470.12435633660327601No Hit
GCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCACCAATCGGCGGT5470.12435633660327601No Hit
CTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTC5460.12412899412319689No Hit
ATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTAAAATTAGTTA5430.12344696668295954No Hit
GTAGAGGAATAGAGTCCCGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5410.12299228172280131No Hit
CCTTAGGAAAAAATCTGGATGGCATAACTAATTTTAATCTTCTCAATTCA5350.12162822684232662No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC5330.12117354188216839No Hit
GGTTAACTCCTTCTTTTCCTGACATTCATTTACATCAAGATTTGATACAC5270.11980948700169369No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5260.11958214452161457No Hit
CAATAGGGCGATTTACAGGAAGTTTACGAAAACAATCTTTGAGTTTTTGT5230.11890011708137725No Hit
CTCTAGGAAGTACACCTCCTTCCCATGGCTGCTCGGTTGTGCTGCCAACT5210.118445432121219No Hit
GGGTTATACCCTAAATTTCATGGGTTATGTTATTGGCAGTTGGGGATCAT5200.1182180896411399No Hit
CATTCATTTACATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCC5190.11799074716106078No Hit
CTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGAC5120.11639934980050698No Hit
GTCCGGCAGATGAGAAGGAACAGACGGGGAGGCCGCGTAAAGAGAGGTGC5110.11617200732042786No Hit
GGCACAGGGTGTGCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCA5090.11571732236026963No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTATTGTTGACAT5080.11548997988019051No Hit
TAAATACACAGTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCC5070.1152626374001114No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGAGACACAGAACCCTGCTCCGA5060.11503529492003228No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCAGGAGGAGTGCGAATCCACACT5010.1138985825196367No Hit
CTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA4930.11207984267900378No Hit
GTAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCCAATGGCCAAG4910.11162515771884557No Hit
GTTATTGGCAGTTGGGGATCATTACCACAAGATCACATTGTACAAAAACT4900.11139781523876643No Hit
GTCCAAGAGTCCGCTTATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTC4890.11117047275868731No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCCACGACCACC4880.11094313027860822No Hit
GGCCTACAAATTGTTCACATTTGTGTATCAAATCTTGATGTAAATGAATG4850.11026110283837087No Hit
GTATAACCCCATCTTTTTGTTTTCGAGGTATTTAAATGGATACCCACAGA4800.1091243904379753No Hit
TGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGATA4690.10662362315710502No Hit
TGTTAGTATTGACATAGTTAACCACTAGGTCCCTAGCAGCAGGATCTTCC4670.1061689381969468No Hit
ACCCAGGACCGTATCCTCCCACTCCTACCTTGTTTGCCATTGGCCAATTG4650.10571425323678856No Hit
CCCATAGGTATTTTCCTAAAGCCCAGGATGATGGGATGGGAATACAAGTG4620.10503222579655122No Hit
CCCCCACACGGTGGCCTGTTGGGGTGGAGTCCACAGGCACAGGGTGTGCT4570.10389551339615563No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT4570.10389551339615563No Hit
ATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAATAA4530.10298614347583918No Hit
ATACAGCCCCAAGCGACCCCGAGAGGGATCGTCCGCTGGATTCAGCGCCG4510.10253145851568096No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTAT4480.10184943107544361No Hit
GCATAACTAATTTTAATCTTCTCAATTCATTTTTTGTGAGAGGGCCTACA4470.10162208859536448No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGAAGCTGGAGATTGAGATCT4440.10094006115512713No Hit
GTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCA4430.10071271867504804No Hit
GTTGTAAGCACACCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTG4420.10048537619496892No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAGCTCCTCCCAGT4420.10048537619496892No Hit
GTTCACATTTGTGTATCAAATCTTGATGTAAATGAATGTCAGGAAAAGAA4400.10003069123481069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)