FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439865
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT26580.6042763120502882No Hit
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT26130.5940459004467279No Hit
GGAAAATATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTT22800.5183408545803826No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT15760.35829174860468554No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15540.35329021404294497No Hit
CCTTTATACCGCTGTTACCAATTTTCTGTTATCTGTGGGTATCCATTTAA13580.3087310879474384No Hit
GGAAAGTATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTT13200.300092073704432No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT12010.2730383185750173No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG11360.25826105736987487No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG11310.2571243449694793No Hit
CTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTT11100.25235015288781787No Hit
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT10710.24348379616473234No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC10600.24098302888386208No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT10560.24007365896354566No Hit
ATACTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAACGTGG10380.23598149432212157No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC10300.23416275448148866No Hit
ATATTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAACGTGG10210.2321166721607766No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA10160.23097995976038102No Hit
AATACTTTCCTATGGAAAAGGGAATTAAACCCTATTATCCTGATAACGTG10030.22802450751935252No Hit
GTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGG9740.22143157559705817No Hit
ATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGAT9610.2184761233560297No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCCGAAAGTCCAA9590.21802143839587143No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT9140.20779102679231126No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC8920.20278949223057077No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATA8910.2025621497504916No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG8650.19665124526843464No Hit
CCATAAAGTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGT8550.1943778204676435No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT8150.18528412126447888No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC7760.17641776454139338No Hit
GTCTTAAAATAATGATTAACCACGTTATCAGGATAATAGGGTTTAATTCC7130.16209518829640912No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTG7080.16095847589601356No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA7030.15982176349561797No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA6750.15345617405340276No Hit
ATTTTAATCTTCTCAATTCATTTTTTGTGAGAGGGCCTACAAATTGTTCA6740.15322883157332365No Hit
ACACAAATGTGAACAATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAA6660.1514100917326907No Hit
ACTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTT6610.15027337933229515No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG6610.15027337933229515No Hit
CTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTGTTCCTACT6530.1484546394916622No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG6510.147999954531504No Hit
GGGTAAAGGTTCATGTATTGTTGACATAGAAAGGCCTTGTAAGTTGGCGA6460.1468632421311084No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC6460.1468632421311084No Hit
GTCTTACATAAGCGGACTCTTGGACTTTCGGGAAGGTCAATCACCTGGAT6340.144135132370159No Hit
GAAAATATTTGGTAACCTTAGGAAAAAATCTGGATGGCATAACTAATTTT6320.1436804474100008No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA6320.1436804474100008No Hit
AATGTGAACAATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTA6320.1436804474100008No Hit
GATTAAAATTAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATAT6290.14299841996976345No Hit
CCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCT6240.1418617075693679No Hit
CCTCTATTCAGGGCAAATTCCAGCAGTCCCGACTGGGACTTCAACAAAAA6180.1404976526888932No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC6180.1404976526888932No Hit
GCTAGGGACCTAGTGGTTAACTATGTCAATACTAACATGGGCCTAAAAAT6170.14027031020881406No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTC6140.1395882827685767No Hit
GTCCCGTGCTGGTGGTCGTGGATCCTGGAAGTAGAGGACAAACGGGCAAC6110.1389062553283394No Hit
CTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTC6040.13731485796778556No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC6010.1366328305275482No Hit
CATTTACATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCCCTCT5950.13526877564707354No Hit
GCCAATAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTT5950.13526877564707354No Hit
TTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTAAAATTAGTTATG5920.1345867482068362No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAGTATTTGGTAACCTT5890.13390472076659884No Hit
ATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA5840.13276800836620328No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5830.13254066588612415No Hit
CTCTAGGAAGTACACCTCCTTCCCATGGCTGCTCGGTTGTGCTGCCAACT5790.1316312959658077No Hit
CCTTAGGAAAAAATCTGGATGGCATAACTAATTTTAATCTTCTCAATTCA5780.1314039534857286No Hit
GGGCTGTATCGCCCCCTTCTCCGTCTGCCGTTCCAGCCGACGACGGGTCG5730.130267241085333No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC5640.12822115876462095No Hit
ATTTGTAGGCCCTCTCACAAAAAATGAATTGAGAAGATTAAAATTAGTTA5620.12776647380446274No Hit
ATCTGTGGGTATCCATTTAAATACCTCGAAAACAAAAAGATGGGGTTATA5620.12776647380446274No Hit
GCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCACCAATCGGCGGT5510.12526570652359245No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGAGACACAGAACCCTGCTCCGA5480.12458367908335512No Hit
GGTTAACTCCTTCTTTTCCTGACATTCATTTACATCAAGATTTGATACAC5390.12253759676264309No Hit
GTTTCCGTCCGAGGGTTTGGAACAGCAGCAAGAGGGAAACATAGAGGTTC5390.12253759676264309No Hit
CAATAGGGCGATTTACAGGAAGTTTACGAAAACAATCTTTGAGTTTTTGT5250.11935480204153548No Hit
CTCTTTACGCGGCCTCCCCGTCTGTTCCTTCTCATCTGCCGGACCGTGTG5240.11912745956145634No Hit
GTATGGATCGGTAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5210.118445432121219No Hit
GTGGTTAACTATGTCAATACTAACATGGGCCTAAAAATTAGACAATTGCT5200.1182180896411399No Hit
CAATATAGCTTGCCTGAGAGCGGTATGGTTAGGTGTGCAATGTTCTGGTG5200.1182180896411399No Hit
CTGTACGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA5190.11799074716106078No Hit
ATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGCAGGTC5060.11503529492003228No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG5050.11480795243995316No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4990.11344389755947848No Hit
CCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCA4980.11321655507939936No Hit
GTATAACCCCATCTTTTTGTTTTCGAGGTATTTAAATGGATACCCACAGA4960.11276187011924113No Hit
AGATAGGGGCATTTGGTGGTCTATAAGCAGGAGGAGTGCGAATCCACACT4950.11253452763916201No Hit
CTCAAGAACTGTTTCTCTTCCAAAAGTAAGGCAGGAAATGTGAAACCACA4910.11162515771884557No Hit
CTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGAC4870.11071578779852909No Hit
TGTTAGTATTGACATAGTTAACCACTAGGTCCCTAGCAGCAGGATCTTCC4850.11026110283837087No Hit
GTTGTAAGCACACCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTG4850.11026110283837087No Hit
GCATAACTAATTTTAATCTTCTCAATTCATTTTTTGTGAGAGGGCCTACA4840.11003376035829174No Hit
ATACAGCCCCAAGCGACCCCGAGAGGGATCGTCCGCTGGATTCAGCGCCG4800.1091243904379753No Hit
GGGTTATACCCTAAATTTCATGGGTTATGTTATTGGCAGTTGGGGATCAT4770.10844236299773795No Hit
GTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCA4740.1077603355575006No Hit
GCCATGGGAAGGAGGTGTACTTCCTAGAGAGGACAACAGAGTTGTCTGTG4730.10753299307742148No Hit
GGCCTACAAATTGTTCACATTTGTGTATCAAATCTTGATGTAAATGAATG4620.10503222579655122No Hit
CCCCCACACGGTGGCCTGTTGGGGTGGAGTCCACAGGCACAGGGTGTGCT4600.10457754083639298No Hit
CATTCATTTACATCAAGATTTGATACACAAATGTGAACAATTTGTAGGCC4580.10412285587623475No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCCACGACCACC4470.10162208859536448No Hit
GTTAATGACTTTGGCTTCCTGGGTGGGCAATAACTTGGAAGATCCTGCTG4460.10139474611528539No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT4460.10139474611528539No Hit
GTATCGCCCCCTTCTCCGTCTGCCGTTCCAGCCGACGACGGGTCGCACCT4440.10094006115512713No Hit
ATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAATAA4440.10094006115512713No Hit
GTACAAAAACTCAAAGATTGTTTTCGTAAACTTCCTGTAAATCGCCCTAT4420.10048537619496892No Hit
GGCACAGGGTGTGCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCA4400.10003069123481069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)