FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459386
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT11880.2586060524264997No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10740.23379032012294673No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9680.21071604271788869No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9530.20745081478321065No Hit
ACCACGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9390.20440326871084447No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAACTCAGACAAATATT9320.20287949567466138No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9290.20222645008772577No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8950.19482526676912226No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8510.18524726482740003No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT8440.18372349179121697No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8380.18241740061734577No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8040.17501621729874223No Hit
TACCGTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7980.17371012612487102No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC7860.1710979437771286No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG7850.17088026191481673No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC7830.17044489819019298No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC7520.16369676045852508No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7210.15694862272685714No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7140.15542484969067408No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA6900.15020048499518923No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6730.14649989333588748No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6710.14606452961126373No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6650.14475843843739253No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6650.14475843843739253No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6640.14454075657508064No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6620.1441053928504569No Hit
CCTCAAGCTTACAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA6480.14105784677809075No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6230.13561580022029404No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA6220.13539811835798216No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5900.1284322987640024No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5830.1269085257278193No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG5760.12538475269163624No Hit
GTATAGGCCCTTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5660.12320793406851754No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5550.120813433583087No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5470.11907197868459204No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG5260.1145006595760428No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5210.11341225026448346No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5190.11297688653985974No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5110.11123543164136478No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT5110.11123543164136478No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5090.11080006791674105No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA5040.10971165860518171No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCAAGC5020.10927629488055796No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5010.10905861301824608No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4980.1084055674313105No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4960.10797020370668675No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT4960.10797020370668675No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4940.10753483998206302No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT4860.10579338508356806No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA4830.10514033949663246No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4800.10448729390969687No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA4720.1027458390112019No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4690.1020927934242663No Hit
ATATAGAGCAGAGGCGGTGTCGAGGAGATCCCGAATAGACGGAAAGAAGT4600.1001336566634595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)