FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459386
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT11050.24053845785461464No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10670.23226654708676364No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10400.22638913680434317No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG9510.2070154510585869No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9360.20375022312390886No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9250.20135572263847834No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8640.18807712903745435No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAACTCAGACAAATATT8510.18524726482740003No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT8460.1841588555158407No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC7590.16522053349470814No Hit
ACCACGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7550.16434980604546068No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7390.16086689624847078No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7310.15912544134997583No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC7230.15738398645148088No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7180.15629557713992157No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7000.1523773036183079No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6860.14932975754594174No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6760.14715293892282308No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6750.14693525706051122No Hit
CCTCAAGCTTACAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA6600.14367002912583318No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6590.14345234726352132No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG6270.1364865276695415No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA6210.13518043649567033No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTGCGTCTGCCG6030.13126216297405666No Hit
TACCGTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6010.13082679924943294No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5640.1227725703438938No Hit
GTATAGGCCCTTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5610.1221195247569582No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCAAGC5540.12059575172077511No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG5510.11994270613383952No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5510.11994270613383952No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5490.11950734240921577No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5440.11841893309765643No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5430.11820125123534458No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA5410.11776588751072083No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5300.11537138702529028No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5260.1145006595760428No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC5210.11341225026448346No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5120.11145311350367665No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5100.1110177497790529No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5090.11080006791674105No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5060.11014702232980543No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5000.10884093115593421No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4970.10818788556899862No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4860.10579338508356806No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4790.104269612047385No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG4780.10405193018507312No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT4770.10383424832276125No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA4700.10231047528657818No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCAAGCTTACAGACCAGCAAATGCC4690.1020927934242663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)