FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605301
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA31160.5147852060379877No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT22720.37535044548084345No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22690.37485482429402894No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19880.328431639795738No Hit
GTGTAAAAGGGGCAGCGAACCCTAAAAGACCTACAATTCGTTGACAGACT19830.32760560448438053No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA19570.3233102208653216No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCGTTTATGCC19030.31438903950266067No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA18340.30298975220592733No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG18210.3008420603963978No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA17430.287955909539221No Hit
CTTTTACACAATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATT17140.28316490473334754No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACATAAATC16590.2740785163084152No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT16270.2687918903157272No Hit
CCCTAAAAGACCTACAATTCGTTGACAGACTTTCCAATCAATAGGCCTGT16020.26466171375893977No Hit
CATATAAAGGCATCAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG15150.2502886993413194No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT14780.244176038037274No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG14100.23294195780281216No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC14040.23195071542918316No Hit
CTCTAAGGCCTCCCGATGCAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA13970.23079426599328265No Hit
GGGTAAAGGTTCAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA13820.2283161600592102No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTA13750.22715971062330972No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT13740.22699450356103826No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGT13670.22583805412513774No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCTAAAAGACCTACAATTCG12800.2114650397075174No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT12770.2109694185207029No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT12640.20882172671117344No Hit
ATGTAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC12070.19940492416169806No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCT11620.19197060635948066No Hit
AGTCTGTACAACATCTTGAGTCCGTTTATGCCGCTGTTACCAATTTTCTT11080.18304942499681975No Hit
GTTCCAGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC11070.18288421793454826No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11060.18271901087227677No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCTAAAAGACCTACAAT10800.17842362725321784No Hit
GGTTCAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA10800.17842362725321784No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTTTG10250.16933723882828544No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT10120.16718954701875596No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC9760.161242092776982No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA9690.16008564334108155No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9680.15992043627881006No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA9600.15859877978063808No Hit
CTTTATATGCATGTATTCAAGCTAAACAGGCTTTTACTTTCTCGCCAACT9590.15843357271836656No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGATACGG9480.1566162950333801No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9400.1552946385352081No Hit
ATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATTCAAGCTAAAC9390.1551294314729366No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9380.1549642244106651No Hit
GTGGATATCCTGCTTTGATGCCTTTATATGCATGTATTCAAGCTAAACAG9350.15446860322385061No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG9340.15430339616157912No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTCTGTCAACGAATTGTAGGTCT9280.15331215378795013No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG9010.14885156310661968No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8970.1481907348575337No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8940.1476951136707192No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACTT8910.1471994924839047No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8840.14604304304800422No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT8780.14505180067437523No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGTTTC8780.14505180067437523No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG8710.14389535123847474No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG8550.14125203824213078No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGAAGGAGATTA8520.14075641705531627No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC8390.1386087252457868No Hit
GCTTTGGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG8060.13315689219082738No Hit
GTACAACATCTTGAGTCCGTTTATGCCGCTGTTACCAATTTTCTTTTGTC8060.13315689219082738No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC7890.1303483721322119No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACACGTAT7860.12985275094539742No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7800.12886150857176842No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7740.12787026619813943No Hit
ATTTATGCCTACAGCCTCCTAATACATAAATCATTAACCTAATCTCCTTC7670.12671381676223897No Hit
GTCTTTGGGTATACATTTAAACCCTCATAAAACAAAAAGATGGGGATATT7640.12621819557542446No Hit
CCTTTATATGCATGTATTCAAGCTAAACAGGCTTTTACTTTCTCGCCAAC7610.12572257438860998No Hit
CTCCAAAAGACACCAAATATTCAAGAACGGTTTCTCTTCCAAAAGTAAGA7600.12555736732633846No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG7590.12539216026406697No Hit
TCATTAACCTAATCTCCTTCCCCAACTCCTCCCACTCAGTAAACACACAG7560.12489653907725247No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7520.12423571082816648No Hit
AATTAAGGGAATATCCCCATCTTTTTGTTTTATGAGGGTTTAAATGTATA7440.1229140543299945No Hit
GTACAATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCC7440.1229140543299945No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7400.1222532260809085No Hit
GAATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGTGTAAAA7230.11944470602229304No Hit
GTTATATGGATGATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7230.11944470602229304No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7210.11911429189775005No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT7180.11861867071093556No Hit
CAGTTATATGGATGATATGGTATTGGGGGCCAAGTCTGTACAACATCTTG7180.11861867071093556No Hit
CCCTTAATTTCATGGGATATGTAATTGGGAGTTGGGGCTCCTTGCCACAG7170.11845346364866406No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGATACGGCAGACGGAGAAGC7140.11795784246184957No Hit
TTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTATGA7040.11630577183913458No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC7030.11614056477686308No Hit
GATCCATACTGCGGAACTCCTAGCCGCCTGTTTTGCTCGCAGCCGGTCTG6970.1151493224032341No Hit
GTTCAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6970.1151493224032341No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG6940.1146537012164196No Hit
CACCGGAACCTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTTTCAGC6930.1144884941541481No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC6930.1144884941541481No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC6920.1143232870918766No Hit
TAAACGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCATATCATC6890.1138276659050621No Hit
TACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTATGAG6780.11201038822007564No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT6680.11035831759736066No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6670.11019311053508916No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA6620.10936707522373167No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG6600.10903666109918866No Hit
CACTTGGCACAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGAT6590.10887145403691717No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6560.10837583285010267No Hit
CTAAAAGACCTACAATTCGTTGACAGACTTTCCAATCAATAGGCCTGTTT6480.1070541763519307No Hit
TCCACATTGTGTAAAAGGGGCAGCGAACCCTAAAAGACCTACAATTCGTT6440.1063933481028447No Hit
CTCTTGGACTTTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA6400.1057325198537587No Hit
GTAGAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCTG6350.10490648454240122No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCAGTGGGCGTTC6310.10424565629331524No Hit
ATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAAACAGG6260.10341962098195773No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCGTTTATGCCGCTGT6200.10242837860832874No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6180.10209796448378576No Hit
CATGTATTCAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6140.10143713623469977No Hit
CACTGGAACCTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTTTCAGC6110.10094151504788527No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6070.10028068679879927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)