FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341278
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG15320.4489008960436946No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC14170.4152040272153493No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT13930.40817163719899907No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCAACCGTG11740.34400107829980253No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11360.3328664607739145No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA11180.3275921682616518No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11140.32642010325892673No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10650.3120623069755449No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA9920.29067212067581266No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA9880.2895000556730876No Hit
GTACGGGACAATGCAAAACCTGCACGACTCCTGCTCAAGGCAAATCTATG9800.28715592566763753No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9750.2856908444142312No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9530.2792444868992434No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT9420.2760213081417495No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT8830.258733349351555No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT8810.2581473168501925No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT8640.25316604058861103No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC8270.24232443931340433No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG7520.22034822051230962No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG7270.21302281424527805No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG7250.21243678174391553No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7220.21155773299187172No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTACCCAGATC7170.21009265173846542No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7060.2068694729809715No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA7010.20540439172756522No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC6800.1992510504632587No Hit
CTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCT6580.19280469294827093No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA6530.1913396116948646No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6420.1881164329373707No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6260.18342817292647048No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC6070.1778608641635265No Hit
GGGTAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5840.17112149039785746No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT5820.17053545789649494No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG5770.16907037664308863No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5740.16819132789104482No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5720.16760529538968233No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5670.166140214136276No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG5640.16526116538423222No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC5630.16496814913355096No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5590.1637960841308259No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTT5540.16233100287741958No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA5510.1614519541253758No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT5510.1614519541253758No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC5460.15998687287196947No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5450.1596938566212882No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5230.15324749910630042No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA5170.15148940160221286No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG5140.15061035285016908No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5090.14914527159676275No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4870.14269891408177499No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATCGA4820.14123383282836866No Hit
GTCCCGTACTGGTCGTTGTTGATCCTGAAATTAGAGGACAAACGGGCAAC4810.1409408165776874No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4780.1400617678256436No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4770.13976875157496235No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4760.1394757353242811No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4670.13683858906814972No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC4660.13654557281746846No Hit
TCCTCAGGCCATGCAGTGGAATTCCGCTGCATTCCACCAAGCTCTGCAGG4490.131564296555887No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG4460.1306852478038432No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC4440.13009921530248067No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA4420.12951318280111815No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4380.12834111779839308No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT4330.1268760365449868No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT4270.1251179390408992No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4200.12306682528613036No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG4190.1227738090354491No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC4160.12189476028340532No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA4150.12160174403272406No Hit
GTACAAAACCTATGGATGCAAATTGCACCTGTATTCCCATCCCATCGTCC4150.12160174403272406No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG4130.12101571153136154No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCG4110.120429679029999No Hit
ACAACAACCAGTACGGGACAATGCAAAACCTGCACGACTCCTGCTCAAGG4070.11925761402727394No Hit
GTTTACTAGCGCCCTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4060.11896459777659268No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT4000.11720650027250512No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3980.1166204677711426No Hit
GCCTAATCATCTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGC3900.11427633776569249No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC3890.11398332151501121No Hit
CCCCCATAGGTATCTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGG3850.11281125651228616No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT3850.11281125651228616No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG3830.11222522401092365No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG3820.11193220776024239No Hit
GTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGGCCTAT3810.11163919150956113No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTCCGCGAAAGGACGACAGAAT3810.11163919150956113No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT3780.11076014275751733No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT3780.11076014275751733No Hit
CTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGT3770.11046712650683607No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3770.11046712650683607No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA3700.10841601275206722No Hit
ATTTATGCCTACAGCCTCCTAATACAAAAATCTTTAACCTAATCTCCTCC3680.1078299802507047No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT3670.10753696400002345No Hit
GTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA3670.10753696400002345No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG3670.10753696400002345No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3650.10695093149866092No Hit
GTATCCCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGACTCA3650.10695093149866092No Hit
CTTCAAAGACTGTGTGTTTAAGGACTGGGAGGAGCTGGGGGAGGAGATTA3640.10665791524797966No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3630.1063648989972984No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAGATACAGACTCCT3620.10607188274661714No Hit
CCCTTAGACAAAGGAATTAAACCTTATTACCCAGATCAGGTAGTTAATCA3580.10489981774389208No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT3580.10489981774389208No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACGGCACTCAGGCA3560.10431378524252954No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA3520.10314172023980449No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT3510.10284870398912323No Hit
CCTCTGCACGTTGCATGGAGACCACCGTGAACGCCCATCAGATCCTGCCC3450.10109060648503566No Hit
CTGCTAGGCTGTACTGCCAACTGGATCCTTCGCGGGACGTCCTTTGTCTA3420.10021155773299187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)