FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284035
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10400.3661520587251571No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC10300.3626313658527998No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9440.3323534071505272No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT8920.31404580421426936No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8130.28623233052264685No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT8080.2844719840864682No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7650.2693330047353319No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7470.2629957575650888No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA6920.2436319467671238No Hit
GGCAACTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACT6350.22356399739468727No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC6000.21124157234143678No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT5980.21053743376696532No Hit
ATATTTGCCTGATCTTTAAACCCATGTTAGTATTAACATAATTGACTACT5900.2077208794690795No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG5900.2077208794690795No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG5850.20596053303290088No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATCAAACCTTATTATCCAGATC5600.19715880085200768No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT5520.19434224655412183No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT5350.18835706867111449No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA5340.18800499938387874No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC5290.18624465294770012No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA5200.18307602936257855No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4920.17321808931997817No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT4850.1707536043093281No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA4660.16406428785184923No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA4540.1598394564050205No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4500.1584311792560776No Hit
GTTAGACGACGGGACCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCC4490.1580791099688419No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT4490.1580791099688419No Hit
TCCCTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4470.15737497139437043No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4460.15702290210713468No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4440.15631876353266322No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT4280.15068565493689157No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG4190.14751703135177002No Hit
GTACGGGTCCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG4180.1471649620645343No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG4060.14294013061770555No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATCAAAC4030.14188392275599837No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC4000.1408277148942912No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA3950.13906736845811254No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT3870.13625081416022675No Hit
GGATAATAAGGTTTGATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT3780.1330821905751052No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC3740.13167391342616225No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG3720.1309697748516908No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG3690.12991356698998363No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT3670.12920942841551217No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT3660.12885735912827645No Hit
GATCATTAACCTAATCTCCTCCCCCAGCTCCTCCCAGTCCTTAAACACAC3650.1285052898410407No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC3620.12744908197933352No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3580.12604080483039062No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3580.12604080483039062No Hit
GACCCGTACTGGTGGTTGTTGTTCCTGGAATTAGAGGACAAACGGGCAAC3520.12392838910697626No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT3490.12287218124526908No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3480.12252011195803335No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA3430.1207597655218547No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC3410.12005562694738325No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA3400.11970355766014752No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3390.11935148837291178No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA3390.11935148837291178No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA3330.11723907264949741No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3270.11512665692608305No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3270.11512665692608305No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATCAAACCTTAT3230.11371837977714014No Hit
CACTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG3230.11371837977714014No Hit
GTCTTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTT3230.11371837977714014No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT3190.11231010262819723No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG3180.11195803334096151No Hit
TACTTCACTCAGGCAAGCCATTGTCTGCTGGGGGGAATTGATGACTCTAG3170.11160596405372578No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA3170.11160596405372578No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTAAACCCTGCTCCGA3160.11125389476649004No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTATAAAGGGCCTC3100.10914147904307568No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC3080.10843734046860423No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3080.10843734046860423No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG3050.10738113260689705No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGATGCGCTACGT3050.10738113260689705No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC2990.10526871688348266No Hit
CTTGTAAATGAATCTTAGGAAAGGAAGGAGTTTGCCATTCAGGATTAAAG2960.10421250902177548No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAG2930.1031563011600683No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA2930.1031563011600683No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT2910.10245216258559685No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA2880.10139595472388965No Hit
TTCCCCCACTGTTTGGCTTTCAGCTATATGGATGATGTGGTATTGGGGGC2870.10104388543665394No Hit
CCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGAGC2860.1006918161494182No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT2850.10033974686218247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)