FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284035
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9940.34995687151231364No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9330.3284806449909342No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC9330.3284806449909342No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT8510.2996109634376045No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8280.2915133698311828No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8130.28623233052264685No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT7260.25560230253313854No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGTCACCCGTG7110.2503212632246026No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7070.24891298607565968No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA6810.23975918460753076No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA6220.21898709666062283No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT6120.21546640378826554No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA6000.21124157234143678No Hit
ATATTTGCCTGATCTTTAAACCCATGTTAGTATTAACATAATTGACTACT5910.20807294875631524No Hit
GGCAACTATTGTGGTTTCATATATCTTGCCTTACTTTTGGAAGAGAGACT5820.2049043251711937No Hit
ATTGATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG5700.20067949372436494No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT5510.1939901772668861No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA5300.18659672223493584No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5010.17638671290509972No Hit
CCTATCAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT4940.17392222789444964No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA4890.172161881458271No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATCAAACCTTATTATCCAGATC4880.17180981217103528No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT4760.16758498072420652No Hit
TCCCTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG4730.16652877286249934No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT4670.16441635713908498No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4650.16371221856461352No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4620.16265601070290633No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC4560.16054359497949197No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT4520.15913531783054907No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4410.15526255567095604No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT4380.15420634780924886No Hit
GTTAGACGACGGGACCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCC4280.15068565493689157No Hit
GTATAAAGGGCCTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC4210.1482211699262415No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG4180.1471649620645343No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG4170.1468128927772986No Hit
GTACGGGTCCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG4130.14540461562835566No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG4110.14470047705388422No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA4090.14399633847941273No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAATGTTCCCCAACTTC3940.13871529917087683No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG3800.13378632914957664No Hit
AGTCTGTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTT3790.13343425986234092No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3790.13343425986234092No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA3780.1330821905751052No Hit
GTCTTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTT3780.1330821905751052No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3770.13273012128786946No Hit
GGATAATAAGGTTTGATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT3750.132025982713398No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG3660.12885735912827645No Hit
GTACTTGAATATTTGGTCTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3650.1285052898410407No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC3630.12780115126656927No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3580.12604080483039062No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3510.12357631981974053No Hit
GGATTAAAGATAGGTACTGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC3500.1232242505325048No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA3490.12287218124526908No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3470.12216804267079762No Hit
TACTTCACTCAGGCAAGCCATTGTCTGCTGGGGGGAATTGATGACTCTAG3420.12040769623461899No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATCAAAC3410.12005562694738325No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC3350.11794321122396886No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGATGCGCTACGT3330.11723907264949741No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA3330.11723907264949741No Hit
CACTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG3300.11618286478779023No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT3290.1158307955005545No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3270.11512665692608305No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA3270.11512665692608305No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT3240.11407044906437587No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATCAAACCTTAT3240.11407044906437587No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA3230.11371837977714014No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTAAACCCTGCTCCGA3190.11231010262819723No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA3160.11125389476649004No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAG3150.11090182547925433No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT3130.11019768690478286No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3120.10984561761754714No Hit
GTACAATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC3110.10949354833031141No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT3080.10843734046860423No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC3070.1080852711813685No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3050.10738113260689705No Hit
GGCCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTTGGGCTTT3040.10702906331966132No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT3010.10597285545795411No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG3010.10597285545795411No Hit
CATATATCTTGCCTTACTTTTGGAAGAGAGACTGTACTTGAATATTTGGT3000.10562078617071839No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA2970.10456457830901121No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT2930.1031563011600683No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG2920.10280423187283257No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT2920.10280423187283257No Hit
GACCCGTACTGGTGGTTGTTGTTCCTGGAATTAGAGGACAAACGGGCAAC2910.10245216258559685No Hit
GTACAGCATCGTGAGGCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC2890.10174802401112538No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT2860.1006918161494182No Hit
GTGGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2850.10033974686218247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)