FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480949
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25960.5397661706334768No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA21470.446409078717286No Hit
CACTCATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT10700.22247681147065487No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10650.22143720020210045No Hit
GTGGACTTACTCTCGTTTTTGCCTTCCGACTTCTTTCCTTCCGTACGAGA10300.21415992132221917No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10160.2112490097702667No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9020.187545872847225No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8950.1860904170712487No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT8850.1840111945341398No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT8310.1727833928337516No Hit
CATCAAACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC8050.1673774142372684No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7930.1648823471927377No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC7710.16030805761109806No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7230.15032778943297523No Hit
GTACATTATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC7020.14596142210504648No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7000.1455455775976247No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6930.14409012182164843No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6570.13660492068805632No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT6440.1339019313898147No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC6400.13307024237497114No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6320.131406864345284No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6200.1289117973007533No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6070.1262088080025117No Hit
CTATGAATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC6030.12537711898766812No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTTAT6030.12537711898766812No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA6010.12496127448024634No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6000.12475335222653545No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6000.12475335222653545No Hit
TCCTAGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTC5860.12184244067458296No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5770.11997114039118492No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5770.11997114039118492No Hit
CCTCCGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGCATTCTTCACCT5760.11976321813747404No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5550.11539685080954529No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5520.1147730840484126No Hit
CCTCAGCTCGGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAA5490.11414931728727994No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC5480.11394139503356905No Hit
ACATAACTGACTACTAGGTCCCTGGATATTGGATCTTCCAAATTACCACC5430.11290178376501459No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5350.11123840573532744No Hit
GAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTTTCCGAAAG5320.11061463897419477No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5310.11040671672048388No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA5310.11040671672048388No Hit
GTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5230.10874333869079673No Hit
ATGCCGAGGCGGTATCTAGCAGATCTCGTACGGAAGGAAAGAAGTCGGAA5190.10791164967595317No Hit
ACACATAGCGCCTCATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCT5160.1072878829148205No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5160.1072878829148205No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5120.10645619389997692No Hit
TCTCCCAATTTTCTAGGGGGAACTACCGTGTGTCTTGGCCAAAATTCGCA5050.10500073812400067No Hit
CAGTCATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGT4990.10375320460173532No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4970.10333736009431353No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT4950.10292151558689176No Hit
ATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTA4910.10208982657204818No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4880.1014660598109155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)